BD 210430 - Difco Tetrathionate Broth Dehydrated Culture Media for Selective Enrichment of Salmonella Species 500 g
The BD 210430 Difco Tetrathionate Broth is a selective enrichment medium formulated for the isolation and recovery of Salmonella species from clinical, food, and environmental samples. Developed under the Difco brand by BD, a global leader in microbiology, this dehydrated medium is specifically designed to suppress competing intestinal flora while promoting the growth of Salmonella organisms.
The formulation includes tetrathionate and bile salts, which inhibit many Gram-positive and non-target Gram-negative bacteria, allowing Salmonella to proliferate more effectively. Iodine solution is typically added during preparation to activate the tetrathionate system, enhancing selectivity. This medium is widely used as part of standard protocols for detecting Salmonella in complex samples where background flora may otherwise obscure results.
Its dehydrated format ensures long shelf life, consistent composition, and ease of preparation, making it a dependable choice for routine microbiological workflows.
| Manufacturer | Application | Color |
| 210430 | Culture Media | White |
Key Benefits
The BD Difco Tetrathionate Broth enhances the selective recovery of Salmonella, improving detection rates in samples with mixed microbial populations. Its formulation effectively suppresses competing organisms, allowing clearer identification of target pathogens.
The medium supports consistent and reproducible results, which is essential for reliable diagnostics and quality control. Its dehydrated form simplifies storage and preparation, enabling laboratories to prepare fresh media as needed while reducing waste.
By improving enrichment efficiency, this product helps laboratories achieve faster and more accurate detection of Salmonella, supporting better decision-making in clinical and food safety applications.
Compatibility and Applications
The BD 210430 Tetrathionate Broth is compatible with standard microbiological laboratory equipment and workflows. It is commonly used in conjunction with selective plating media such as Hektoen Enteric Agar, XLD Agar, or SS Agar following enrichment.
Typical applications include clinical microbiology laboratories, food safety testing facilities, environmental monitoring programs, and public health laboratories. It is widely used for detecting Salmonella in stool samples, food products, water samples, and other materials requiring pathogen screening.
Usage Guidelines
To prepare the medium, dissolve the specified amount of dehydrated powder in purified water according to the manufacturers instructions. Heat gently with agitation to ensure complete dissolution.
After cooling to the appropriate temperature, add iodine solution as directed to activate the tetrathionate components. Dispense into sterile containers and inoculate with the sample.
Incubate under recommended conditions and subculture onto selective agar media for isolation and identification of Salmonella. Refer to the product insert for exact preparation steps and incubation parameters.
Certifications and Safety Standards
The BD 210430 Difco Tetrathionate Broth is manufactured under strict quality control systems aligned with internationally recognized standards such as ISO certification and Good Manufacturing Practices. These standards ensure consistent quality, safety, and performance.
The product meets regulatory expectations for microbiological culture media used in clinical, food safety, and research environments, supporting reliable diagnostic outcomes.
Comparison with Competitor Products
Compared to other selective enrichment broths, the BD Difco Tetrathionate Broth offers superior consistency and selectivity due to its carefully balanced formulation. Competing products may exhibit variability in inhibitory performance, which can affect Salmonella recovery.
BDs advanced manufacturing processes ensure uniform composition and dependable results across batches. Its compatibility with established laboratory protocols and reliable enrichment performance make it a preferred choice over generic alternatives.
The Difco brand is widely recognized for its high-quality culture media, providing additional assurance of performance and reliability.
Typical User Profile and Application Scenarios
The BD 210430 Tetrathionate Broth is used by microbiologists, laboratory technicians, and quality control specialists in clinical, food, and environmental testing laboratories. It is also utilized in research settings focused on pathogen detection and microbiological analysis.
Common scenarios include screening stool samples for Salmonella, testing food products for contamination, and conducting environmental monitoring. It is particularly valuable in situations where selective enrichment is required to detect low levels of pathogens.
Best Practices for Use and Maintenance
Store the dehydrated medium in a cool, dry environment, protected from moisture and direct sunlight. Ensure the container is tightly sealed after each use to maintain product integrity.
Prepare the medium using sterile techniques and follow recommended guidelines for dissolution and iodine addition. Avoid overheating, as this may affect the mediums performance.
Inspect prepared media for contamination before use and discard any compromised batches. Proper handling and adherence to protocols will ensure reliable results.
Regulatory and Safety Information
The BD 210430 Difco Tetrathionate Broth is intended for professional laboratory use. Users should follow standard safety protocols, including the use of appropriate personal protective equipment when handling samples and reagents.
All used materials should be disposed of according to biohazard waste regulations. Compliance with recognized standards ensures safe and effective use in laboratory environments.
How to Order
Ordering the BD 210430 Difco Tetrathionate Broth is simple through CIA Medical. Locate the product on the website, add it to your cart, and proceed through the secure checkout process.
For bulk orders, pricing inquiries, or assistance with product selection, contact CIA Medical customer service at (312) 275-5850. Their team is available to support your laboratory needs with expert guidance.
