BD 210950 - Difco Sabouraud Dextrose Agar 500g Dehydrated Culture Medium for Fungal and Yeast Isolation,EA
The BD 210950 Difco Sabouraud Dextrose Agar 500g is a high-quality dehydrated culture medium specifically formulated for the cultivation of fungi, yeasts, and molds. Originally developed by Raymond Sabouraud, this medium is optimized to support the growth of dermatophytes, pathogenic, and non-pathogenic fungi. Manufactured by BD (Becton Dickinson) under the trusted Difco brand, the formulation contains peptones and dextrose that provide the necessary nutrients for fungal proliferation while maintaining an acidic pH to inhibit bacterial growth. This 500g bottle is ideal for laboratories requiring reliable, large-scale preparation of consistent, high-performance fungal media.
Key Benefits
The BD Difco Sabouraud Dextrose Agar (SDA) offers superior selectivity and growth support for a broad spectrum of yeasts and molds. Its high dextrose concentration promotes vigorous fungal growth, while its slightly acidic pH suppresses bacterial contamination. The mediums proven formulation ensures reproducible results, batch-to-batch consistency, and dependable performance in clinical, pharmaceutical, and research environments. Its dehydrated form allows for flexible preparation volumes and long shelf life, optimizing cost efficiency and storage convenience for laboratories of all sizes.
Compatibility and Applications
BD 210950 Difco Sabouraud Dextrose Agar is compatible with standard microbiological equipment, including Petri dishes, culture tubes, and flasks. It is widely used in hospitals, diagnostic laboratories, pharmaceutical industries, cosmetic testing facilities, and environmental monitoring programs. Typical applications include the isolation of Candida species, Aspergillus species, Trichophyton species, and other dermatophytes from clinical specimens, as well as contamination testing in sterile manufacturing environments. The medium is also used for teaching and research purposes where fungal culture and identification are required.
Usage Guidelines
To prepare the medium, suspend 65 g of BD Difco Sabouraud Dextrose Agar in 1 liter of purified water. Heat with frequent agitation until the medium is completely dissolved. Sterilize by autoclaving at 121C for 15 minutes. Once sterilized, allow the medium to cool to 4550C before pouring into sterile Petri dishes or tubes. For optimal performance, incubate inoculated plates at 2530C for up to 7 days, depending on the fungal species. Store the dehydrated medium in a tightly closed container at room temperature, away from moisture, heat, and direct sunlight.
Certifications, Safety Standards, and Regulatory Approvals
The BD 210950 Difco Sabouraud Dextrose Agar complies with ISO 11133 and ISO 13485 standards for microbiological media quality and safety. It is manufactured following Good Manufacturing Practices (GMP) and carries CE marking for in vitro diagnostic (IVD) applications. The product meets FDA and international regulatory standards for clinical and industrial use, ensuring traceability, reliability, and safety in microbial culture testing.
Comparison with Competitor Products
Compared to other fungal media, BDs Difco Sabouraud Dextrose Agar provides unmatched consistency, superior clarity, and uniform solidification. Many competitor products may exhibit uneven pH balance or variability in fungal growth. BDs Difco formulation is known for its reproducibility and precise composition, making it the preferred choice for accurate fungal detection and identification. Its dehydrated format allows greater flexibility than pre-poured plates, ensuring cost-effective use in laboratories requiring custom preparation volumes.
Typical User Profile and Application Scenarios
The BD 210950 SDA is used by clinical microbiologists, mycologists, pharmaceutical quality control teams, and research scientists. It is particularly essential in diagnostic laboratories testing for fungal infections, such as candidiasis, aspergillosis, and dermatophytosis. Industrial users rely on it for environmental monitoring and sterility validation in cleanrooms and production facilities. Academic and teaching institutions also utilize this medium for training students in fungal isolation and identification techniques.
Best Practices for Use and Maintenance
Use high-quality distilled or deionized water for media preparation to avoid interference from impurities. Follow aseptic procedures throughout the preparation and inoculation processes to minimize contamination. Do not overheat the medium during sterilization, as excessive heating can alter its nutrient composition. For prolonged storage, keep prepared plates or tubes sealed to prevent dehydration. Validate media performance periodically using control organisms such as Candida albicans ATCC 10231 and Aspergillus niger ATCC 16404 to ensure consistent growth response.
Regulatory and Safety Information
This medium is intended for professional laboratory use only and should not be used for direct human or veterinary diagnosis without proper confirmatory testing. Personnel handling the product should wear appropriate protective equipment, including gloves and lab coats. Dispose of all used cultures and media by autoclaving before discarding, in compliance with institutional biosafety guidelines. Refer to the products Safety Data Sheet (SDS) for detailed hazard and handling instructions. BD ensures compliance with all applicable global safety and performance regulations for diagnostic and industrial microbiological media.
How to Order
To purchase the BD 210950 Difco Sabouraud Dextrose Agar 500g, add the product to your cart and complete the secure checkout process on the CIA Medical website. For bulk orders or recurring supply needs, contact CIA Medical customer service at (312) 275-5850 or use the online contact form for inquiries. CIA Medical provides dependable delivery, competitive pricing, and professional support for all BD Difco microbiological media and laboratory products.
