BD 211065 - Brain Heart Infusion (BHI) Agar Enriched Medium for Cultivation of Fastidious and Non-Fastidious Microorganisms

BD 211065 - Brain Heart Infusion (BHI) Agar Enriched Medium for Cultivation of Fastidious and Non-Fastidious Microorganisms

The BD 211065 Brain Heart Infusion (BHI) Agar is a high-quality, nutrient-rich microbiological medium formulated for the cultivation of a wide range of fastidious and non-fastidious microorganisms. Developed by BD (Becton Dickinson), this classic medium contains infusions of brain and heart tissues that provide amino acids, peptides, and growth factors essential for robust bacterial and fungal growth. It supports aerobic, microaerophilic, and certain anaerobic organisms, making it a versatile medium for clinical, industrial, and research applications. BD BHI Agar is trusted worldwide for its consistency, performance, and ability to recover delicate and demanding microbial species.

Key Benefits

The BD Brain Heart Infusion Agar offers superior nutritional support, enabling the recovery of a broad spectrum of microorganisms, including streptococci, pneumococci, staphylococci, and yeasts. Its enriched composition makes it suitable for cultivating pathogens from sterile body fluids, blood cultures, and other clinical specimens. The mediums well-balanced formula ensures rapid, luxuriant colony growth while maintaining differential morphology and pigment production. Each batch is rigorously tested for sterility, performance, and pH stability, guaranteeing consistent results. The products quality, reproducibility, and broad application make it an essential tool in both diagnostic and research microbiology.

Compatibility and Applications

BD 211065 BHI Agar is compatible with routine microbiological workflows and various laboratory instruments used for culture incubation and analysis. It is widely used in clinical diagnostic laboratories, research institutions, and quality control departments in the pharmaceutical, food, and cosmetic industries. The medium is suitable for isolating fastidious bacteria such as Streptococcus pneumoniae, Neisseria species, Haemophilus species, and Candida species. Additionally, it is used for testing bacterial contamination, maintaining microbial stock cultures, and studying microbial physiology and antibiotic susceptibility.

Usage Guidelines

To prepare the medium, suspend 52 g of BD Brain Heart Infusion Agar in 1 liter of purified water. Heat with frequent agitation until the medium is completely dissolved, then sterilize by autoclaving at 121C for 15 minutes. After sterilization, cool the medium to 4550C and pour into sterile Petri dishes or tubes as required. Once solidified, the agar plates are ready for inoculation. Inoculate the plates with the desired specimen using aseptic technique and incubate at 3537C for 1848 hours, depending on the organism. Store the dehydrated medium in a tightly closed container at room temperature in a dry, cool place away from light and moisture.

Certifications, Safety Standards, and Regulatory Approvals

The BD 211065 Brain Heart Infusion Agar complies with ISO 11133 and ISO 13485 standards for microbiological media performance and quality control. It is produced under Good Manufacturing Practices (GMP) and meets FDA and CE requirements for in vitro diagnostic (IVD) products. Each batch undergoes performance testing using reference strains such as Staphylococcus aureus ATCC 6538, Streptococcus pneumoniae ATCC 6303, and Escherichia coli ATCC 25922 to confirm medium quality and organism recovery efficiency.

Comparison with Competitor Products

Compared to other BHI formulations, BDs 211065 medium offers exceptional clarity, consistent performance, and superior organism recovery. While competitor brands may vary in nutrient balance or yield uneven growth, BDs precisely standardized brain and heart extracts ensure reproducible colony morphology and density. Its optimized peptone and dextrose concentrations support both aerobic and microaerophilic growth, making it a preferred choice for laboratories that demand dependable, high-quality culture results.

Typical User Profile and Application Scenarios

This medium is widely used by microbiologists, laboratory technicians, and quality assurance professionals in clinical diagnostics, pharmaceutical production, food safety, and academic research. It is particularly useful for recovering organisms from sterile sites, maintaining control cultures, and conducting microbial contamination studies. Research facilities also use it for cultivating fungal isolates and for antimicrobial efficacy studies involving complex organisms requiring enriched nutrient environments.

Best Practices for Use and Maintenance

Use sterile technique throughout media preparation and inoculation to prevent contamination. Always verify the performance of each prepared batch using known control strains. Avoid overheating the medium to prevent caramelization and loss of nutrient quality. Store dehydrated powder and prepared media in airtight containers at controlled room temperature. Dispose of all used culture materials following institutional biosafety and environmental regulations. Regular quality control testing ensures ongoing compliance and reliability of laboratory results.

Regulatory and Safety Information

The BD 211065 Brain Heart Infusion Agar is intended for professional laboratory use only. It should be handled by trained personnel following standard biosafety level 2 (BSL-2) precautions. Wear appropriate personal protective equipment (PPE) such as gloves, lab coat, and eye protection. Treat all specimens and used plates as potentially infectious and dispose of them by autoclaving or incineration. Refer to the Safety Data Sheet (SDS) for detailed information regarding handling, storage, and safety guidelines. BD ensures all BHI media comply with global standards for quality, safety, and performance.

How to Order

To purchase the BD 211065 Brain Heart Infusion Agar, add the product to your cart and proceed through the secure checkout process on the CIA Medical website. For institutional or high-volume orders, contact CIA Medical customer service at (312) 275-5850 or submit a bulk inquiry through the contact form. CIA Medical provides reliable delivery, competitive pricing, and professional customer support for all BD microbiological culture media and diagnostic reagents.