BD 211116 - Coagulase Mannitol Agar 500g Differential Medium for Staphylococcal Isolation and Identification, EA
The BD 211116 Coagulase Mannitol Agar (500g) is a specialized differential and selective culture medium formulated for the isolation and identification of coagulase-positive Staphylococcus aureus from clinical and environmental samples. Manufactured by BD (Becton Dickinson), this medium combines the fermentable carbohydrate mannitol with peptones, sodium chloride, and other selective agents to promote the growth of staphylococci while inhibiting the growth of most non-staphylococcal organisms. The presence of coagulase activity and mannitol fermentation enables reliable differentiation of pathogenic S. aureus from other staphylococcal species based on colony appearance and color change in the medium.
| Cat. no. | Quantity |
| 211116 | 500 g |
Key Benefits
BD Coagulase Mannitol Agar provides a rapid and accurate means to distinguish coagulase-positive S. aureus from coagulase-negative staphylococci (CoNS). The mediums high sodium chloride concentration selectively favors staphylococcal growth, while mannitol serves as a differential substrate for fermentation detection. The inclusion of pH indicators allows for clear visualization of fermentation reactions, producing a color change that aids in colony differentiation. Its high-quality formulation ensures consistent results, excellent batch-to-batch reproducibility, and robust colony morphology suitable for confirmatory biochemical testing.
Compatibility and Applications
The BD 211116 Coagulase Mannitol Agar is compatible with standard bacteriological laboratory techniques and can be used in conjunction with confirmatory tests such as the coagulase test or latex agglutination assays. It is widely used in hospital microbiology laboratories, food safety testing, and environmental monitoring to detect and enumerate Staphylococcus aureus. The medium is applicable for clinical samples (e.g., wound swabs, nasal cultures, skin infections), food samples (dairy, meat, bakery products), and quality control monitoring in pharmaceutical and cosmetic industries.
Usage Guidelines
To prepare the medium, suspend 110g of BD Coagulase Mannitol Agar in 1 liter of distilled water. Heat with frequent stirring until the medium is completely dissolved, then sterilize by autoclaving at 121C for 15 minutes. After autoclaving, cool to 4550C, mix gently, and pour into sterile Petri dishes. Once solidified, inoculate the surface of the agar plate with the test sample using a sterile loop or swab. Incubate the plates aerobically at 3537C for 2448 hours. Typical Staphylococcus aureus colonies will appear yellow due to mannitol fermentation, while coagulase-negative species produce pink to red colonies without a color change in the medium.
Certifications, Safety Standards, and Regulatory Approvals
The BD 211116 Coagulase Mannitol Agar complies with ISO 13485 and ISO 9001 quality management standards and meets FDA and CE regulatory requirements for in vitro diagnostic (IVD) use. It is manufactured under Good Manufacturing Practices (GMP) and validated according to CLSI and ISO 11133 guidelines for culture media performance testing. Each batch undergoes rigorous quality control with reference strains such as Staphylococcus aureus ATCC 25923 (positive) and Staphylococcus epidermidis ATCC 12228 (negative) to ensure consistent performance and differentiation accuracy.
Comparison with Competitor Products
Compared to other mannitol-based media, BD Coagulase Mannitol Agar delivers superior clarity, selectivity, and color differentiation. While some competitor products may exhibit variability in color contrast or inhibitory strength, BDs precise formulation ensures optimal balance between selectivity and growth performance. Its robust nutrient base supports healthy colony morphology without overgrowth of contaminants. BDs high purity ingredients and strict quality control provide unmatched reproducibility, making it the trusted choice in clinical and industrial microbiology laboratories.
Typical User Profile and Application Scenarios
This product is used by microbiologists, clinical laboratory scientists, and quality control technicians in medical, food, and industrial testing environments. It is ideal for clinical diagnostic laboratories screening for S. aureus infections, food safety labs monitoring contamination, and pharmaceutical quality departments ensuring compliance with microbial standards. The BD 211116 medium is also suitable for educational laboratories teaching microbial differentiation and biochemical testing principles.
Best Practices for Use and Maintenance
Store the dehydrated medium at 1030C in a tightly closed container, protected from moisture and light. Reconstitute only the amount needed for immediate use to prevent contamination and degradation. Verify the pH (expected range: 7.4 0.2 at 25C) and appearance of the medium after preparation to ensure quality. Avoid overheating or prolonged autoclaving, which may alter the mediums selective properties. Perform routine quality control using recommended reference strains to confirm media performance. Dispose of used plates and cultures according to institutional biosafety and environmental safety regulations.
Regulatory and Safety Information
This product is intended for professional laboratory use only. Handle all biological materials under Biosafety Level 2 (BSL-2) precautions. Use appropriate personal protective equipment (PPE), including gloves, lab coats, and eye protection, during preparation and handling. Avoid inhalation of the dehydrated powder. In case of contact with skin or eyes, rinse thoroughly with water. Refer to the Safety Data Sheet (SDS) for detailed hazard and first-aid information. BD ensures full compliance with global laboratory safety, quality, and environmental standards for culture media production.
How to Order
To purchase the BD 211116 Coagulase Mannitol Agar (500g), add the item to your cart and complete the secure checkout process on the CIA Medical website. For institutional or bulk orders, contact CIA Medical customer service at (312) 275-5850 or use the online inquiry form. CIA Medical offers competitive pricing, fast shipping, and professional support for all BD culture media and microbiology laboratory reagents.
