BD 211221 - Levine Eosin Methylene Blue Agar Selective Differential Medium for Enteric Bacteria
The BD 211221 Levine Eosin Methylene Blue Agar is a selective and differential microbiological medium designed for the isolation and differentiation of Gram-negative enteric bacteria. This medium incorporates eosin Y and methylene blue dyes, which inhibit Gram-positive organisms while allowing differentiation based on lactose fermentation.
Levine formulation enhances the identification of coliforms, particularly Escherichia coli, which produces characteristic metallic green sheen colonies. The medium supports clear differentiation between lactose fermenters and non-fermenters, making it an essential tool in clinical diagnostics, food testing, and water quality analysis.
| Manufacturer | 211221 |
| Application | Dehydrated Culture Media |
| Container Type | Bottle |
| Storage Requirements | Requires Refrigeration |
| Volume | 500 Gram |
Key Benefits
Selective inhibition of Gram-positive bacteria enhances recovery of enteric Gram-negative organisms.
Differential properties allow clear identification based on lactose fermentation.
Characteristic colony appearance supports rapid identification of Escherichia coli.
Consistent formulation ensures reliable and reproducible results.
Compatibility and Applications
BD Levine Eosin Methylene Blue Agar is compatible with standard laboratory equipment, including Petri dishes and incubators. It integrates seamlessly into routine microbiology workflows.
Common applications include detection of coliform bacteria in clinical specimens, food safety testing, and water quality monitoring. It is widely used in hospitals, diagnostic laboratories, public health institutions, and research facilities.
Usage Guidelines
Reconstitute the dehydrated medium using distilled or deionized water according to manufacturer instructions. Heat to dissolve completely and sterilize as recommended.
Pour into sterile Petri dishes and allow to solidify. Inoculate samples using aseptic techniques and incubate under appropriate conditions.
Observe colony morphology and color changes to differentiate organisms based on lactose fermentation.
Certifications and Safety Standards
The BD 211221 Levine Eosin Methylene Blue Agar is manufactured under ISO-certified quality systems, ensuring consistent performance and reliability.
Each batch undergoes rigorous quality control testing to meet microbiological standards, supporting its use in professional laboratory environments.
Comparison with Competitors
Compared to general-purpose media, EMB Agar provides selective and differential capabilities, improving identification accuracy.
BDs stringent manufacturing processes ensure consistent dye concentration and performance, whereas lower-quality alternatives may show variability.
The trusted BD brand ensures dependable results, making this medium a preferred choice for enteric bacteria analysis.
Typical User Profile and Application Scenarios
This product is intended for microbiologists, laboratory technicians, and quality control professionals involved in microbial testing and analysis.
It is commonly used in clinical laboratories, food testing facilities, water testing laboratories, and research institutions where accurate detection of enteric bacteria is essential.
Best Practices for Use and Maintenance
Store the dehydrated medium in a cool, dry environment to prevent moisture absorption and maintain stability.
Prepare only the required quantity to maintain freshness and reduce waste. Use aseptic techniques during preparation and inoculation.
Monitor incubation conditions carefully to ensure optimal growth and accurate interpretation of results.
Regulatory and Safety Information
The BD Levine Eosin Methylene Blue Agar is intended for laboratory use and complies with applicable standards for microbiological media.
Users should follow all relevant biosafety procedures when handling microbial cultures and dispose of used media in accordance with local regulations.
How to Order
To order the BD 211221 Levine Eosin Methylene Blue Agar, add the product to your cart and proceed through CIA Medicals secure checkout process.
For bulk orders, institutional pricing, or additional assistance, contact CIA Medical customer service at (312) 275-5850. Their team is available to support your procurement needs.
