BD 211393 - MacConkey Agar Without Crystal Violet, Dehydrated Culture Medium
The BD 211393 MacConkey Agar Without Crystal Violet is a dehydrated culture medium formulated for the selective and differential isolation of Gram-negative enteric bacilli. This formulation omits crystal violet, allowing improved recovery of certain Gram-positive organisms while maintaining selectivity through bile salts and lactose fermentation indicators. The medium differentiates lactose-fermenting organisms from non-lactose fermenters based on colony color changes.
Manufactured to the quality standards of BD, this dehydrated medium is designed to support consistent, reliable microbiological testing in clinical and research laboratories.
Key Benefits
Selective formulation supports isolation of Gram-negative organisms. Lactose fermentation differentiation allows visual identification of coliforms based on colony color. Crystal violet-free formulation may enhance recovery of specific organisms sensitive to traditional MacConkey components. Dehydrated powder format supports flexible batch preparation according to laboratory needs. Engineered for consistent lot-to-lot performance.
Compatibility and Applications
BD 211393 MacConkey Agar is compatible with standard laboratory media preparation equipment, including autoclaves and sterile Petri dishes. It is widely used in hospital microbiology laboratories, academic research facilities, public health laboratories, and pharmaceutical quality control settings. Typical applications include identification of Enterobacteriaceae, differentiation of lactose-fermenting organisms such as Escherichia coli, and general screening for enteric pathogens.
Usage Guidelines
Prepare the medium according to manufacturer instructions by dissolving the appropriate amount of dehydrated powder in purified water. Heat and sterilize as directed, typically by autoclaving. Pour into sterile Petri dishes under aseptic conditions and allow to solidify. Inoculate specimens according to laboratory protocol and incubate at the recommended temperature. Interpret colony morphology and color changes based on established microbiological guidelines.
Certifications, Safety Standards, and Regulatory Approvals
BD culture media products are manufactured under regulated quality systems designed to ensure consistency, reliability, and compliance with laboratory standards. Quality control testing is performed to verify performance characteristics, supporting use in clinical diagnostic and research applications.
Comparison with Competitor Products
Compared with traditional MacConkey agar containing crystal violet, this formulation may provide enhanced recovery of organisms that are sensitive to crystal violet while retaining selective properties. BD culture media are widely trusted for reproducible performance, rigorous quality control, and dependable differentiation characteristics.
Typical User Profile and Application Scenarios
This product is commonly used by microbiologists, clinical laboratory scientists, and research professionals involved in bacterial isolation and identification. It is particularly effective in diagnostic laboratories processing stool, urine, and other clinical specimens for enteric pathogen screening.
Best Practices for Use and Maintenance
Store the dehydrated medium in a cool, dry environment protected from moisture. Prepare media using calibrated equipment and validated sterilization procedures. Perform routine quality control with known control strains to verify medium performance. Dispose of used culture plates in accordance with biosafety and laboratory waste protocols.
Regulatory and Safety Information
While culture media are laboratory products rather than direct patient-use devices, they are manufactured under strict quality controls to support clinical laboratory compliance. Proper handling, preparation, and disposal in accordance with biosafety guidelines are essential to maintaining laboratory safety and regulatory adherence.
How to Order
Ordering the BD 211393 MacConkey Agar Without Crystal Violet is simple. Add the product to your cart and proceed through the secure checkout process. For bulk laboratory purchasing or additional assistance, contact CIA Medical customer service at (312) 275-5850 or use the contact form available on this page.
