BD 211438 - Difco Mueller Hinton Broth, 500 g Bottle, Dehydrated Culture Medium for Antimicrobial Susceptibility Testing,EA
The BD 211438 - BD Difco Mueller Hinton Broth is a high-quality, dehydrated culture medium designed for the cultivation of a wide range of non-fastidious microorganisms and for performing standardized antimicrobial susceptibility testing (AST). Manufactured by Becton Dickinson (BD) under the Difco brand, this 500 g bottle of Mueller Hinton Broth is formulated according to the specifications of the Clinical and Laboratory Standards Institute (CLSI) and the European Committee on Antimicrobial Susceptibility Testing (EUCAST).
Composed of beef extract, casein hydrolysate, and starch, Mueller Hinton Broth provides optimal growth conditions for aerobic and facultatively anaerobic bacteria. Its nutrient-rich, non-selective composition ensures reliable and reproducible results when used for broth microdilution and antibiotic sensitivity testing protocols.
Key Benefits
The BD Difco Mueller Hinton Broth is known for its consistency, performance, and broad usability in microbiological testing. Major benefits include:
- Standardized formulation: Meets CLSI and EUCAST specifications for accurate and reproducible antimicrobial susceptibility testing.
- Optimal bacterial growth: Supports a wide range of aerobic and facultative organisms without inhibitory substances.
- Excellent reproducibility: Provides consistent performance across different batches, ensuring reliable comparative results.
- High clarity: Produces clear, particle-free solutions ideal for optical density readings and turbidity measurements.
- Convenient format: The 500 g bottle enables flexible preparation of customized volumes for routine or large-scale testing.
Compatibility and Applications
The BD 211438 Mueller Hinton Broth is widely used in both clinical and industrial microbiology laboratories. Applications include:
- Antimicrobial susceptibility testing (AST) using broth microdilution or tube dilution methods.
- General cultivation of bacteria such as Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa.
- Preparation of inocula for Kirby-Bauer disk diffusion assays.
- Microbiological research and bacterial physiology studies requiring standardized nutrient media.
The broth is compatible with automated systems and manual testing procedures, ensuring versatility across various laboratory setups.
Usage Guidelines
To prepare the medium, suspend 21 g of BD Difco Mueller Hinton Broth powder in 1 liter of purified water. Mix thoroughly to dissolve completely. Dispense into appropriate containers and sterilize by autoclaving at 121C for 15 minutes. After sterilization, allow the medium to cool to room temperature before use. Store prepared broth at 28C and use within the recommended shelf life. For antimicrobial susceptibility testing, follow CLSI or EUCAST protocols for inoculum preparation and incubation parameters (35 2C for 1620 hours).
Certifications, Safety Standards, and Regulatory Approvals
The BD Difco Mueller Hinton Broth is manufactured under ISO 13485:2016 and ISO 9001:2015-certified quality management systems. It complies with CLSI M07 and EUCAST standards for antimicrobial susceptibility testing and meets FDA and CE requirements for in vitro diagnostic (IVD) use. Each batch undergoes rigorous quality control for pH (7.3 0.1 at 25C), sterility, and growth performance using reference strains such as Escherichia coli (ATCC 25922), Staphylococcus aureus (ATCC 29213), and Pseudomonas aeruginosa (ATCC 27853).
Comparison with Competitor Products
Compared to other commercially available media, BD Difco Mueller Hinton Broth offers superior clarity, batch consistency, and compliance with international AST guidelines. Competitor products may exhibit variable pH or inconsistent cation content, which can affect antibiotic diffusion and result interpretation. BDs formulation ensures optimal calcium and magnesium ion concentrations, minimizing test variability and ensuring reliable antibiotic susceptibility results across bacterial species.
Typical User Profile and Application Scenarios
This product is intended for professional use by microbiologists, clinical laboratory technologists, and researchers in:
- Clinical microbiology and diagnostic laboratories performing AST testing.
- Pharmaceutical quality control and antibiotic development labs.
- Public health and food testing laboratories conducting microbial analysis.
- Academic and industrial research institutions studying bacterial resistance patterns.
It is ideal for laboratories requiring a validated, reproducible medium for bacterial growth and antibiotic efficacy studies.
Best Practices for Use and Maintenance
Store the dehydrated BD Difco Mueller Hinton Broth powder tightly closed in a cool, dry environment (225C). Protect from moisture and direct sunlight. Use sterile glassware and distilled water during preparation to maintain media quality. Avoid overheating or over-autoclaving, as this may alter the pH and nutrient composition. Discard any prepared broth that appears cloudy, discolored, or contaminated. Follow biosafety and waste disposal procedures for all bacterial cultures and materials handled.
Regulatory and Safety Information
The BD 211438 - BD Difco Mueller Hinton Broth is an FDA-cleared and CE-marked in vitro diagnostic medium for professional laboratory use. It is non-toxic, DEHP-free, and latex-free. A Safety Data Sheet (SDS) is available from BD upon request, providing detailed information on composition, handling, and disposal. The product contains no hazardous chemicals under normal laboratory use and complies with international biosafety and environmental safety regulations.
How to Order
To order the BD 211438 - BD Difco Mueller Hinton Broth (500 g Bottle), add the product to your cart and complete checkout through CIA Medical. We offer competitive pricing, fast delivery, and comprehensive support for clinical, pharmaceutical, and research laboratories. For bulk purchases, Certificates of Analysis, or product validation data, contact CIA Medicals customer service team at (312) 275-5850 or use the contact form on this page. Our team can assist with product availability, documentation, and technical specifications.
