BD 211665 - Nutrient Agar 2.2lb General-Purpose Culture Medium for Non-Fastidious Microorganisms, EA

BD 211665 - Nutrient Agar 2.2lb General-Purpose Culture Medium for Non-Fastidious Microorganisms, EA

The BD 211665 Nutrient Agar (2.2lb / 1kg) is a high-quality, general-purpose culture medium formulated for the isolation, cultivation, and maintenance of non-fastidious microorganisms. Manufactured by BD (Becton Dickinson), this medium provides a balanced nutrient base containing peptones and beef extract to support the robust growth of bacteria and fungi. It is widely used in routine laboratory testing, teaching, and microbial enumeration across clinical, industrial, and environmental microbiology applications. Known for its clarity, consistency, and reproducibility, BD Nutrient Agar is an essential component in microbiological testing and quality control programs.

Key Benefits

BD Nutrient Agar offers dependable growth performance for a wide range of non-fastidious organisms such as Escherichia coli, Bacillus subtilis, and Staphylococcus aureus. The mediums balanced formulation provides essential nitrogen, vitamins, and carbon sources for microbial metabolism. It produces a firm, clear gel that allows easy observation of colony morphology and pigmentation. The product is easy to prepare and sterilize, ensuring convenience for routine culture work. BDs stringent manufacturing standards guarantee uniform composition and high reproducibility across batches, making it a reliable choice for microbial culture and maintenance.

Compatibility and Applications

The BD 211665 Nutrient Agar is compatible with standard laboratory procedures and equipment used for microbial culture, including Petri dishes, test tubes, and slant preparations. It is used for the cultivation, enumeration, and maintenance of bacteria from clinical, environmental, food, and water samples. This medium is also suitable for teaching laboratories, industrial microbiology quality control, and basic microbial identification. It can be used as a base for media supplementation when specific growth factors or selective agents are required for specialized microorganisms.

Usage Guidelines

To prepare the medium, suspend 23g of BD Nutrient Agar in 1 liter of distilled water. Heat with continuous stirring until the agar is completely dissolved, then sterilize by autoclaving at 121C for 15 minutes. Cool the sterilized medium to approximately 4550C before pouring into sterile Petri dishes or culture tubes. Once solidified, inoculate the agar surface with the desired microbial sample using aseptic technique. Incubate plates at the appropriate temperature for the microorganism being studied, typically 3037C for bacteria and 25C for fungi. Store prepared plates at 28C and use within a few weeks for best results.

Certifications, Safety Standards, and Regulatory Approvals

The BD 211665 Nutrient Agar is produced under ISO 13485 and ISO 9001 quality management systems and meets FDA and CE regulatory standards for in vitro diagnostic (IVD) media. It is manufactured under Good Manufacturing Practices (GMP) and validated according to CLSI and ISO 11133 performance guidelines for culture media. Each batch undergoes strict quality control testing using standard reference strains such as Staphylococcus aureus ATCC 25923 and Escherichia coli ATCC 25922 to verify consistent growth characteristics, pH, and gel firmness.

Comparison with Competitor Products

Compared to generic nutrient agars, BD Nutrient Agar stands out for its superior batch consistency, clarity, and nutrient balance. While some competitor products may vary in peptone quality or gel strength, BD ensures optimal protein digest and agar purity to achieve consistent microbial recovery. Its transparency facilitates clear colony visualization, and its formulation supports a broad spectrum of microbial species. Laboratories prefer BD Nutrient Agar for its reproducibility, longevity, and proven reliability in both clinical and industrial microbiological testing.

Typical User Profile and Application Scenarios

This product is ideal for microbiologists, laboratory technologists, educators, and researchers conducting microbial isolation and identification. It is widely used in hospitals, research institutions, environmental monitoring facilities, and industrial microbiology laboratories. The BD 211665 Nutrient Agar is particularly useful for general bacteriology teaching, routine sterility testing, and colony morphology studies. It serves as a reliable base medium for microbial culture maintenance and basic laboratory diagnostics.

Best Practices for Use and Maintenance

Store the dehydrated medium in a tightly sealed container at 1030C, protected from moisture and direct sunlight. Always use freshly prepared media for optimal results and confirm the pH (expected value 6.8 0.2 at 25C) before sterilization. Avoid overheating, which can cause agar degradation or caramelization of nutrients. Use aseptic techniques during preparation and inoculation to prevent contamination. Dispose of used culture materials according to institutional biosafety and environmental regulations. For long-term microbial preservation, subculture periodically on freshly prepared BD Nutrient Agar plates or slants.

Regulatory and Safety Information

This product is intended for professional laboratory use only. Handle all microbial cultures as potentially infectious and follow Biosafety Level 1 or 2 precautions, depending on the organism. Use appropriate personal protective equipment (PPE) such as gloves, lab coats, and safety goggles during media preparation and handling. Avoid inhalation of the dehydrated powder and contact with eyes. In case of accidental exposure, rinse thoroughly with water. Refer to the Safety Data Sheet (SDS) for detailed safety and handling instructions. BD ensures compliance with all relevant laboratory safety and environmental standards.

How to Order

To purchase the BD 211665 Nutrient Agar (2.2lb), add the product to your cart and complete the secure checkout process on the CIA Medical website. For bulk or institutional orders, contact CIA Medical customer service at (312) 275-5850 or use the online inquiry form. CIA Medical offers competitive pricing, reliable shipping, and expert support for all BD Difco and BBL microbiological media products.