BD 211744 - Lauryl Tryptose Broth with MUG Dehydrated Culture Media for Coliform and E. coli Detection
The BD 211744 Lauryl Tryptose Broth with MUG is a specialized dehydrated microbiological culture medium designed for the selective detection and enumeration of coliform bacteria and Escherichia coli in water, food, and environmental samples. Manufactured by BD, a globally trusted leader in microbiology, this medium combines the traditional Lauryl Tryptose Broth formulation with the addition of MUG, a fluorogenic substrate that enables rapid identification of E. coli.
The formulation supports the growth of coliform organisms while inhibiting non-target bacteria. The presence of MUG allows E. coli to produce a fluorescent compound when hydrolyzed by the enzyme beta-glucuronidase, enabling easy confirmation under ultraviolet light. This dual-function capability makes the medium highly effective for both presumptive and confirmatory testing.
The dehydrated format ensures long shelf life and consistent performance, allowing laboratories to prepare fresh media as needed while maintaining quality and reliability.
Key Benefits
The BD 211744 medium provides efficient and reliable detection of coliforms and E. coli, supporting accurate microbiological analysis. The inclusion of MUG enables rapid confirmation of E. coli through fluorescence, reducing the need for additional confirmatory tests.
Its selective formulation improves specificity by suppressing non-coliform organisms, ensuring clearer and more accurate results. The medium supports consistent microbial growth, allowing for dependable interpretation across different testing environments.
The dehydrated format simplifies storage and preparation, improving workflow efficiency and reducing waste in laboratory operations.
Compatibility and Applications
The BD Lauryl Tryptose Broth with MUG is compatible with standard microbiological testing systems, including multiple-tube fermentation methods and presence-absence testing protocols. It integrates easily into routine laboratory workflows.
Typical applications include water quality testing, food safety analysis, environmental monitoring, and quality control in industrial settings. It is widely used in public health laboratories, municipal water testing facilities, food processing plants, and research institutions.
Usage Guidelines
To prepare the medium, dissolve the appropriate amount of dehydrated powder in purified water according to the manufacturers instructions. Mix thoroughly and sterilize by autoclaving at the recommended conditions.
After sterilization, dispense into sterile tubes or containers as required. Inoculate the medium with the sample and incubate under specified conditions.
Observe for gas production as an indication of coliform presence. For E. coli confirmation, examine the culture under ultraviolet light for fluorescence resulting from MUG hydrolysis.
Refer to the product insert for exact preparation details, incubation times, and interpretation guidelines.
Certifications and Safety Standards
The BD 211744 medium is manufactured in compliance with internationally recognized quality standards, including ISO-certified production processes and Good Manufacturing Practices. These standards ensure consistency, safety, and reliability.
The product is suitable for laboratory use and aligns with regulatory expectations for microbiological culture media used in clinical, environmental, and industrial testing.
Comparison with Competitor Products
Compared to standard Lauryl Tryptose Broth formulations without MUG, the BD 211744 offers enhanced functionality by enabling direct detection of E. coli through fluorescence. This reduces the need for additional confirmatory media or biochemical tests.
BDs formulation ensures consistent nutrient composition and performance, providing more reliable results than many generic alternatives. Its clarity and selectivity also improve interpretation compared to lower-quality media that may produce ambiguous results.
The integration of both presumptive and confirmatory testing capabilities makes it a more efficient solution for modern laboratories.
Typical User Profile and Application Scenarios
The BD 211744 medium is commonly used by microbiologists, laboratory technicians, environmental health professionals, and quality control specialists. It is also utilized in educational and research settings.
Typical scenarios include testing drinking water for contamination, monitoring food safety in production environments, conducting environmental assessments, and performing routine quality control checks in laboratories.
Best Practices for Use and Maintenance
Store the dehydrated medium in a cool, dry place, protected from moisture and direct sunlight. Ensure the container is tightly sealed after each use to maintain product integrity.
Prepare the medium using sterile techniques and follow recommended guidelines to ensure consistent results. Avoid overheating during preparation, as this may degrade essential components.
Inspect prepared media for contamination before use and discard any compromised samples. Proper handling and adherence to protocols will ensure reliable performance.
Regulatory and Safety Information
The BD 211744 Lauryl Tryptose Broth with MUG is intended for professional laboratory use. Users should follow standard safety protocols, including the use of appropriate personal protective equipment when handling samples and reagents.
All used materials should be disposed of according to biohazard waste regulations. Compliance with recognized standards ensures safe and effective use in laboratory environments.
How to Order
Ordering the BD 211744 Lauryl Tryptose Broth with MUG is simple through CIA Medical. Locate the product on the website, add it to your cart, and proceed through the secure checkout process.
For bulk orders, pricing inquiries, or assistance with product selection, contact CIA Medical customer service at (312) 275-5850. Their team is available to support your laboratory needs with expert guidance.
