BD 211795 - MD Agar Urea Base Differential Medium for Rapid Urease Detection, 500G
The BD 211795 MD Agar Urea Base is a high-quality differential culture medium designed to detect urease activity in microorganisms. This medium serves as the base formulation for preparing urea agar by incorporating a sterile urea solution. Once supplemented, the medium supports rapid identification of urease-positive organisms through clear and immediate color reactions. Its composition includes essential nutrients, peptones, and a pH indicator that responds to ammonia production, enabling accurate biochemical differentiation of clinically relevant bacteria.
Key Benefits
BD MD Agar Urea Base provides fast and reliable urease detection, producing distinctive color changes that simplify interpretation. Its stable formulation ensures predictable performance, and the mediums high clarity enhances visibility of reactions. Because urea is added separately, the medium offers extended shelf life and greater stability prior to supplementation. BDs stringent quality controls ensure consistent reproducibility, supporting dependable microbial identification in clinical and research workflows.
Compatibility and Applications
This medium is widely used for biochemical differentiation of urease-producing organisms, including:
- Proteus species
- Klebsiella species
- Helicobacter pylori (in specialty workflows)
- Yersinia species
Typical applications include:
- Clinical microbiology identification panels
- Differential testing of Enterobacterales
- Verification of urease activity in environmental and research isolates
- Educational teaching laboratories focusing on biochemical reactions
Usage Guidelines
Rehydrate the dehydrated MD Agar Urea Base according to the manufacturers instructions. Sterilize the medium by autoclaving at 121C for 15 minutes. After cooling to 5055C, aseptically add sterile-filtered urea solution to complete the formulation. Pour into sterile tubes or plates as required. Inoculate using a straight stab or streak method and incubate at 3537C. Urease-positive organisms produce a distinct color shift (typically from yellowish to deep pink or red), indicating alkaline pH due to ammonia release. Store dehydrated medium in a cool, dry location.
Certifications, Safety Standards, and Regulatory Approvals
The BD 211795 MD Agar Urea Base is manufactured in ISO 9001 and ISO 13485 certified facilities and conforms to FDA and CE standards for in vitro diagnostic (IVD) media. Each batch undergoes rigorous quality assurance testing for pH accuracy, sterility, ingredient consistency, and performance using known control organisms.
Comparison with Competitor Products
Compared with general urea agar products, BDs MD Agar Urea Base provides superior flexibility because urea is added after sterilization, preventing degradation from autoclaving. Competitor media may show inconsistent urease reactions or reduced shelf life due to premature urea breakdown. BDs high-grade raw materials and reliable manufacturing result in sharper color changes and more reproducible biochemical differentiation.
Typical User Profile and Application Scenarios
This medium is widely used by:
- Clinical microbiologists and diagnostic technologists
- Research scientists studying bacterial metabolism
- Public health laboratories
- Academic institutions teaching microbial identification
It is ideal for routine ID workflows, confirmatory biochemical panels, and organism screening in both clinical and industrial settings.
Best Practices for Use and Maintenance
Always use sterile technique when supplementing with urea. Avoid overheating urea-containing media, as urea rapidly breaks down at high temperatures. Prepare only the amount needed for short-term use to maintain optimal performance. Check all tubes or plates for contamination or dehydration before testing. Dispose of inoculated media as regulated biohazard waste.
Regulatory and Safety Information
The BD 211795 MD Agar Urea Base is intended for in vitro diagnostic use by trained laboratory personnel. While the medium itself is non-hazardous, cultures grown within it may contain pathogenic microbes requiring biosafety level 2 precautions. Wear gloves, lab coat, and protective eyewear. Follow institutional guidelines for waste handling and decontamination.
How to Order
To order the BD 211795 MD Agar Urea Base, add the product to your cart and complete checkout through CIA Medicals secure online platform. For large-volume orders, product documentation, or technical assistance, contact CIA Medical customer service at (312) 275-5850 or submit an inquiry through the contact form on this page. Our team is ready to support your microbiology media requirements.
