BD 212218 - CLED Agar BBL Bottle 500g Differential Medium for Urinary Pathogen Isolation,EA
The BD 212218 CLED Agar BBL Bottle 500g is a high-quality dehydrated culture medium formulated for the isolation, differentiation, and enumeration of urinary pathogens. Cystine-Lactose-Electrolyte-Deficient (CLED) Agar is specifically designed to support the growth of common urinary tract bacteria while preventing the swarming of Proteus species. Manufactured by BD (Becton Dickinson), this premium-grade medium ensures consistent performance in clinical microbiology laboratories. The 500g bottle provides ample material for multiple laboratory preparations, offering both cost efficiency and high reliability in diagnostic testing.
Key Benefits
The BD CLED Agar BBL medium provides excellent differentiation between lactose-fermenting and non-lactose-fermenting organisms, helping laboratory professionals quickly identify causative agents of urinary tract infections (UTIs). Its electrolyte-deficient formulation prevents overgrowth by motile Proteus species, maintaining discrete colonies for accurate enumeration. The product ensures consistent batch quality, dependable results, and enhanced laboratory efficiency. Additionally, BDs strict quality control processes and packaging integrity support reliable shelf stability and contamination-free usage, improving diagnostic accuracy and patient outcomes.
Compatibility and Applications
The BD 212218 CLED Agar BBL medium is compatible with a variety of culture systems and standard microbiological workflows. It is widely used in hospital and reference laboratories for urine culture and sensitivity testing. Suitable for use with common urinary pathogens such as Escherichia coli, Klebsiella spp., Proteus spp., Enterococcus spp., and Pseudomonas aeruginosa. The medium can be used with Petri dishes, inoculating loops, and automated streaking systems, making it versatile for both manual and automated microbiological testing environments.
Usage Guidelines
To prepare the medium, suspend 36 g of BD CLED Agar in 1 liter of purified water. Heat with frequent agitation until completely dissolved, then sterilize by autoclaving at 121C for 15 minutes. Allow the medium to cool to 4550C before pouring into sterile Petri dishes. Once solidified, the plates are ready for inoculation. For best results, incubate inoculated plates at 3537C for 1824 hours. Store the dehydrated medium at room temperature in a tightly sealed container, away from moisture and direct sunlight. Always follow laboratory biosafety and aseptic handling protocols.
Certifications, Safety Standards, and Regulatory Approvals
The BD 212218 CLED Agar BBL Bottle 500g complies with ISO 11133 standards for microbiological media quality and performance. It is produced in accordance with ISO 13485-certified manufacturing processes and meets Good Manufacturing Practice (GMP) guidelines. The product carries CE marking for in vitro diagnostic (IVD) use and adheres to FDA standards for clinical diagnostic media. BDs adherence to these regulatory standards ensures superior consistency, traceability, and safety for laboratory use.
Comparison with Competitor Products
When compared to other dehydrated CLED agars on the market, BDs formulation offers superior reproducibility, color clarity, and batch-to-batch consistency. Unlike lower-grade alternatives that may exhibit uneven colony morphology or contamination risks, BD 212218 CLED Agar provides uniform media quality for precise bacterial growth and differentiation. Its extended shelf life and optimized formulation make it a preferred choice among laboratory professionals seeking dependable results and ease of preparation in routine diagnostics.
Typical User Profile and Application Scenarios
This product is widely used by clinical microbiologists, laboratory technicians, and healthcare professionals conducting urine culture tests. It is particularly suited for clinical microbiology labs, diagnostic centers, research institutions, and hospital laboratories where UTI diagnosis and surveillance are routine. The BD CLED Agar BBL medium is also used in teaching and research environments for demonstrating microbial growth characteristics and biochemical differentiation.
Best Practices for Use and Maintenance
For optimal performance, always use high-quality distilled or deionized water when preparing the medium. Avoid overheating during sterilization to prevent nutrient degradation. After preparation, store plates in sealed packaging or plastic bags to prevent desiccation. Regularly validate the mediums performance using known control strains such as E. coli (lactose fermenter) and P. aeruginosa (non-lactose fermenter). Maintain proper aseptic conditions throughout preparation and inoculation to prevent contamination. Dispose of all used media according to institutional biosafety regulations.
Regulatory and Safety Information
This culture medium is intended for professional laboratory use only and should not be used for direct clinical diagnosis without proper confirmation. Always review the products Safety Data Sheet (SDS) before use. The medium should be handled by trained personnel wearing appropriate personal protective equipment (PPE). All waste should be autoclaved before disposal to ensure biosafety compliance. BD ensures that the product meets or exceeds all applicable safety and performance requirements for diagnostic microbiology media.
How to Order
To order the BD 212218 CLED Agar BBL Bottle 500g, add the item to your cart and follow the secure checkout process on the CIA Medical website. For larger quantities or institutional supply agreements, contact CIA Medical customer service at (312) 275-5850 or submit a bulk order inquiry through the contact form. CIA Medical provides reliable delivery, transparent pricing, and excellent support for all BD microbiology and diagnostic media products.
