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BD 212351 - Difco Middlebrook OADC Enrichment, 500 mL, Refrigerated Perishable Laboratory Supplement
The BD 212351 Difco Middlebrook OADC is a prepared enrichment supplement supplied in a 500 mL format for professional microbiology laboratory use. OADC enrichment is commonly used with Middlebrook media formulations to support the cultivation and recovery of mycobacteria and other organisms requiring enriched growth conditions.
This product is intended for trained laboratory professionals working in microbiology, clinical research, public health, pharmaceutical quality control, academic research, and specialized culture-based testing environments. It is used as a supplement in validated laboratory media preparation workflows and should be handled according to manufacturer instructions and facility standard operating procedures.
BD 212351 is a perishable product that must be refrigerated. It is non-returnable and available for Continental US shipment only. Buyers should confirm current availability, refrigerated receiving capability, storage requirements, and lead time before placing an order.
Key Benefits
BD 212351 Difco Middlebrook OADC supports reliable enriched media preparation by providing a ready-to-use supplement designed for Middlebrook culture media workflows. It helps laboratories prepare media that can support the growth of organisms with more demanding nutritional requirements, including mycobacteria when used according to validated methods.
The 500 mL format is practical for laboratories that perform recurring media supplementation but need a manageable container size for controlled use and refrigerated storage. It helps reduce preparation complexity compared with sourcing individual enrichment components separately.
As part of the BD Difco microbiology product portfolio, this supplement is suitable for laboratories that prioritize recognized media components, consistent sourcing, lot traceability, and standardized preparation procedures.
Compatibility and Applications
BD 212351 Difco Middlebrook OADC is designed for use with compatible Middlebrook media formulations according to BD instructions and validated laboratory protocols. It may be used in culture media preparation workflows where oleic acid, albumin, dextrose, and catalase enrichment is required.
Common applications include mycobacterial culture support, enriched media preparation, research culture workflows, clinical microbiology support, public health laboratory testing, pharmaceutical microbiology, academic research, and specialized organism recovery procedures.
This product is typically used in microbiology laboratories, reference laboratories, clinical research facilities, university laboratories, pharmaceutical quality control departments, biotechnology facilities, and public health or specialized testing laboratories. It is not intended for direct patient use, injection, administration, or therapeutic application.
Usage Guidelines
Use BD 212351 Difco Middlebrook OADC only according to BD product instructions, validated laboratory methods, and facility standard operating procedures. Before use, confirm the correct base medium, supplementation ratio, handling requirements, storage temperature, expiration date, and quality control expectations.
Maintain refrigerated storage as required and return the product to proper storage promptly after use. Use aseptic technique when adding the supplement to prepared media to reduce contamination risk and preserve media integrity.
Do not use the product if the container is damaged, expired, contaminated, improperly stored, or if the supplement shows unexpected discoloration, precipitate, leakage, or other signs of compromise. Because this product is perishable and non-returnable, receiving staff should inspect the shipment promptly upon arrival and document any temperature or damage concerns according to facility policy.
Certifications and Safety Standards
BD 212351 Difco Middlebrook OADC is manufactured for professional laboratory use and should be handled in accordance with applicable laboratory safety standards, institutional quality procedures, and manufacturer guidance. Facilities should maintain documentation for lot number, expiration date, receiving condition, refrigerated storage records, preparation records, and quality control results.
Regulatory and compliance requirements may vary depending on the laboratorys intended use, industry sector, and validation requirements. Buyers should verify current product labeling, certificate of analysis availability, safety data sheet, quality specifications, and compliance documentation through BD, CIA Medical, or authorized product documentation before use.
Comparison with Competitors
Compared with generic OADC enrichment products, BD 212351 Difco Middlebrook OADC offers the advantage of being part of BDs established Difco microbiology media and supplement portfolio. This may be important for laboratories that require recognized sourcing, lot traceability, and consistent documentation for validated workflows.
Compared with preparing enrichment from individual components, the ready-to-use OADC format helps reduce formulation complexity and supports more standardized media preparation. This can improve workflow efficiency and reduce variability in laboratories that routinely prepare Middlebrook media.
Compared with larger-volume or bulk enrichment options, the 500 mL size provides a practical balance between supply availability and storage control. It is well suited for laboratories that need dependable refrigerated enrichment without excessive unused volume.
Typical User Profile and Application Scenarios
The typical users of BD 212351 Difco Middlebrook OADC include microbiologists, laboratory technicians, clinical laboratory scientists, research scientists, media preparation specialists, public health laboratory staff, pharmaceutical quality control analysts, and laboratory procurement teams.
This product is most useful in laboratory environments where Middlebrook media is prepared for enriched organism cultivation. Common scenarios include mycobacterial culture support, research media preparation, clinical microbiology workflows, specialized organism recovery, public health testing support, and quality control procedures requiring enriched culture conditions.
Best Practices for Use and Maintenance
Store BD 212351 Difco Middlebrook OADC under refrigerated conditions according to manufacturer recommendations. Keep the container closed when not in use and minimize time outside refrigeration to help maintain product quality.
Use aseptic technique when handling the supplement. Avoid repeated unnecessary exposure to room temperature, open-air contamination, or contact with non-sterile instruments. Record the lot number, opening date, expiration date, storage conditions, and use history according to facility policy.
Before adding the supplement to media, confirm that the base medium has been prepared according to validated instructions and cooled to the appropriate temperature if required. Mix gently and thoroughly after supplementation to ensure uniform distribution.
Perform applicable quality control checks on prepared media, such as appearance, sterility testing, growth promotion testing, pH verification, or method-specific acceptance criteria. Do not use prepared media or supplement that shows contamination, leakage, unexpected turbidity, discoloration, or other signs of compromise.
Regulatory and Safety Information
BD 212351 Difco Middlebrook OADC is intended for professional laboratory use only. It should not be used for direct patient administration, clinical treatment, injection, food use, or any application outside the intended laboratory environment.
Personnel should follow standard laboratory safety practices, including appropriate personal protective equipment, aseptic handling, labeling, storage documentation, spill management, and disposal procedures. Prepared media and cultures should be handled according to applicable biosafety requirements, institutional protocols, and federal, state, and local regulations.
Facilities should review the current BD safety data sheet, product labeling, certificate of analysis, and relevant quality documentation before use. Compliance requirements may differ depending on whether the product is used for research, clinical laboratory support, public health work, pharmaceutical quality control, academic work, or industrial microbiology workflows.
How to Order
Select the BD 212351 Middlebrook OADC 500 mL and add it to your cart. Complete checkout with your preferred shipping and payment options. This product is perishable, must be refrigerated, non-returnable, and available for Continental US shipment only. For bulk orders, quotes, availability checks, or further assistance, contact CIA Medical customer service at (312) 275-5850 or use the contact form on this page.