BD 212519 - TB Fluorescent Stain Kit 25mL Diagnostic Staining System for Acid-Fast Bacilli (AFB) Detection, 4/BX
The BD 212519 TB Fluorescent Stain Kit (25mL) is a high-performance diagnostic staining system developed by BD (Becton Dickinson) for the rapid and accurate detection of Mycobacterium tuberculosis and other acid-fast bacilli (AFB) in clinical specimens. This kit uses auramine O fluorescent dye, which binds specifically to mycolic acids in the mycobacterial cell wall, allowing AFB to fluoresce under ultraviolet (UV) illumination. Designed for clinical microbiology and reference laboratories, the BD TB Fluorescent Stain Kit provides a fast, sensitive, and reliable alternative to traditional Ziehl-Neelsen staining methods, improving detection rates and laboratory efficiency.
| Manufacturer | Application | Container Type |
| 212519 | Acid Fast Bacteria (AFB) Stain Kit | Bottle |
Key Benefits
The BD TB Fluorescent Stain Kit offers several critical advantages for tuberculosis diagnostics. It enables faster screening of slides due to its high sensitivity and clear fluorescent signal, significantly reducing examination time compared to carbol-fuchsin stains. The auramine O fluorescent dye produces bright yellow-green bacilli against a dark background, making AFB easier to detect even at low concentrations. The kit provides consistent staining results across batches, ensuring reproducibility and reliability. It is easy to use, requires minimal reagent preparation, and integrates seamlessly into existing laboratory staining workflows.
Compatibility and Applications
This staining kit is compatible with standard fluorescence microscopy systems equipped with UV or blue light excitation filters. It is intended for use in the identification of Mycobacterium tuberculosis complex and other acid-fast organisms such as Mycobacterium avium and Mycobacterium kansasii in sputum smears, tissue samples, and other clinical materials. The BD 212519 TB Fluorescent Stain Kit is widely used in hospital microbiology laboratories, public health institutions, and reference centers performing tuberculosis (TB) diagnosis, surveillance, and research.
Usage Guidelines
Prepare the smear on a clean, grease-free slide and heat-fix the specimen. Flood the slide with BD TB Fluorescent Stain and allow it to stain for the recommended time (usually 1520 minutes). Rinse gently with distilled water and apply the acid-alcohol decolorizer until the background appears clear. Counterstain using the provided potassium permanganate or methylene blue solution for 12 minutes, then rinse and air-dry. Examine the slide under a fluorescence microscope at 400x magnification. Acid-fast bacilli will appear as bright yellow-green rods against a dark background. Always follow BDs detailed product insert instructions for optimal performance.
Certifications, Safety Standards, and Regulatory Approvals
The BD 212519 TB Fluorescent Stain Kit complies with ISO 13485 and ISO 9001 quality management standards for in vitro diagnostic (IVD) products. It is CE-marked and FDA-cleared for use in diagnostic laboratories. The kit is manufactured under Good Manufacturing Practices (GMP) and meets CLSI guidelines for acid-fast bacilli staining procedures. BDs quality assurance process ensures reagent stability, uniform dye concentration, and reliable performance across multiple staining batches.
Comparison with Competitor Products
Compared to traditional Ziehl-Neelsen (ZN) and Kinyoun acid-fast stains, the BD TB Fluorescent Stain Kit offers superior sensitivity, speed, and visualization. The fluorescence-based method allows rapid scanning of large smear areas with lower magnification, increasing throughput and reducing technician fatigue. Competitor fluorescent kits may show variable intensity or fading over time, while BDs optimized formulation ensures long-lasting fluorescence and stable signal quality. Its ready-to-use format minimizes preparation errors and enhances consistency in routine TB diagnostics.
Typical User Profile and Application Scenarios
This product is designed for clinical microbiologists, laboratory technologists, and TB program specialists conducting acid-fast staining for diagnosis or surveillance. It is particularly valuable in high-volume testing environments such as hospital microbiology departments, national TB control laboratories, and reference centers. The BD 212519 kit is also used in research facilities studying Mycobacterium pathogenesis, drug resistance, and epidemiological patterns. Its ease of use and reproducibility make it ideal for both routine and confirmatory TB testing.
Best Practices for Use and Maintenance
Store the reagents at 28C in their original tightly sealed containers. Avoid prolonged exposure to light to prevent dye degradation. Always mix reagents gently before use to ensure homogeneity. Handle all biological specimens under Biosafety Level 2 (BSL-2) or higher conditions. Decontaminate work surfaces after use and dispose of staining waste according to biohazard and chemical safety regulations. Regularly check fluorescence microscope filters and light sources for optimal viewing conditions. Always follow BDs staining protocol for consistent and reliable results.
Regulatory and Safety Information
This product is intended for professional laboratory use only. The staining reagents contain fluorescent dyes and acids that should be handled with care. Use personal protective equipment (PPE) such as gloves, lab coats, and eye protection while handling reagents and clinical specimens. Avoid inhalation, ingestion, or contact with skin and eyes. In case of contact, rinse thoroughly with water and seek medical advice if irritation persists. Refer to the products Safety Data Sheet (SDS) for detailed hazard and safety information. BD ensures full compliance with global environmental, health, and safety standards.
How to Order
To purchase the BD 212519 TB Fluorescent Stain Kit (25mL), add the product to your cart and complete the secure checkout process on the CIA Medical website. For institutional or high-volume laboratory orders, contact CIA Medical customer service at (312) 275-5850 or submit an inquiry via the online form. CIA Medical provides competitive pricing, fast shipping, and dedicated support for all BD TB diagnostic and microbiology staining products.
