BD 212523 - TB Brilliant Green, 250mL Differential Medium for Mycobacterium tuberculosis Isolation
The BD 212523 TB Brilliant Green medium is a specialized culture medium formulated for the selective isolation and differentiation of Mycobacterium tuberculosis and other mycobacterial species. Supplied in a 250mL format, this medium incorporates brilliant green dye to inhibit contaminating organisms, increasing the likelihood of isolating target mycobacteria from complex clinical specimens. BDs precise formulation ensures consistent colony development, predictable selectivity, and reliable performance in tuberculosis diagnostic workflows.
| Manufacturer | Application | Container Type |
| 212523 | Acid Fast Bacteria (AFB) Stain | Bottle |
Key Benefits
The inclusion of brilliant green provides enhanced selectivity by suppressing the growth of Gram-positive and Gram-negative organisms commonly found in respiratory and tissue samples. The medium supports robust growth of M. tuberculosis while maintaining clear, interpretable colony morphology. BDs controlled production environment ensures consistent pH, dye concentration, and nutrient availability for dependable recovery of clinically significant isolates.
Compatibility and Applications
BD TB Brilliant Green medium is compatible with:
- Standard mycobacterial culture workflows
- Specimen processing protocols for sputum, bronchial washings, tissue, and aspirates
- Incubation in specialized mycobacteriology incubators
Common application scenarios include:
- Primary isolation of M. tuberculosis complex organisms
- Differentiation of mycobacterial species based on colony appearance
- Public health evaluation of suspected TB cases
- Research studies involving mycobacterial growth characteristics
Usage Guidelines
Inoculate the medium using decontaminated and concentrated clinical samples. Incubate at 3537C in a controlled environment, typically for 36 weeks depending on organism load. Observe colony morphology at regular intervals. Avoid excessive exposure to light as brilliant green dye may degrade. Store unused medium at recommended refrigerated conditions and do not use if contamination or drying is observed.
Certifications, Safety Standards, and Regulatory Approvals
This medium is manufactured under ISO 9001 and ISO 13485 certified quality systems. BD mycobacteriology media comply with FDA and CE specifications for in vitro diagnostic (IVD) products. Each lot is tested for sterility, selectivity, mycobacterial recovery performance, and pH accuracy before release.
Comparison with Competitor Products
Compared with generic TB selective media, BD TB Brilliant Green offers more consistent dye concentration, improved selectivity, and reduced contamination rates. Competitor media may show variable inhibitory effects or inconsistent colony visibility. BDs tightly controlled formulation supports reliable detection of slow-growing mycobacteria even in heavily contaminated specimens.
Typical User Profile and Application Scenarios
This product is essential for:
- Clinical microbiology laboratories performing TB diagnostics
- Public health TB control programs
- Reference laboratories specializing in mycobacterial identification
- Academic and research labs studying Mycobacterium species
Best Practices for Use and Maintenance
Use only properly decontaminated samples to reduce competing flora. Label all culture containers clearly for long incubation periods. Inspect plates frequently for contamination or overgrowth. Store medium upright and tightly sealed to prevent dehydration. Follow biosafety level 3 precautions where required for handling M. tuberculosis.
Regulatory and Safety Information
For in vitro diagnostic use only. Handle all suspected TB specimens under appropriate biosafety conditions (BSL-2 enhanced or BSL-3 depending on local regulations). Wear PPE and follow institutional and governmental guidelines for TB culture handling and waste disposal.
How to Order
To order BD 212523 TB Brilliant Green (250mL), add the item to your cart and complete checkout through CIA Medicals secure ordering system. For bulk supply, public health contracts, or technical assistance, contact CIA Medical customer service at (312) 275-5850 or submit an inquiry via the contact form on this page.
