BD 212525 - BD Gram Crystal Violet Reagent, Primary Stain for Gram Staining Procedures, 4/BX

BD 212525 - BD Gram Crystal Violet Reagent, Primary Stain for Gram Staining Procedures, 4/BX

BD 212525 Gram Crystal Violet Reagent is a high-quality, ready-to-use primary stain designed for use in Gram staining procedures. Formulated for use in microbiological laboratories, this reagent serves as the first step in differentiating Gram-positive from Gram-negative organisms by staining bacterial cell walls a distinctive purple. It is manufactured to BDs stringent quality standards, ensuring consistent results in clinical, research, and teaching environments.

Key Benefits

• Reliable primary stain for accurate differentiation in Gram staining

• Produces vivid purple coloration in Gram-positive organisms for easy microscopic identification

• Pre-formulated and ready to use, streamlining workflow in busy labs

• Stable and consistent dye concentration for reproducible results across batches

• Supplied in a laboratory-grade reagent bottle with secure cap to prevent leakage or contamination

• Compatible with manual and automated staining systems

Compatibility and Applications
BD Gram Crystal Violet Reagent is compatible with standard Gram staining protocols and works effectively with:

• Clinical microbiology labs

• Research laboratories

• University and teaching laboratories

• Manual slide staining stations or automated slide stainers

• Bacterial identification in patient samples, water testing, and research cultures

Usage Guidelines
Apply the reagent as the primary stain in a typical four-step Gram staining procedure:

1. Flood the fixed bacterial smear with crystal violet for 3060 seconds.

2. Rinse gently with water.
   Follow up with Grams iodine, decolorizer, and counterstain. Store tightly capped at room temperature, away from light and heat.

Certifications, Safety Standards, and Regulatory Approvals
Manufactured under ISO-certified quality systems and compliant with applicable laboratory reagent regulations. Safety Data Sheets (SDS) are available for safe handling guidance. Intended for in vitro diagnostic use or research applications as labeled.

Comparison with Competitor Products
Unlike lower-grade stains, BDs crystal violet reagent offers superior consistency in coloration and longer shelf stability. The precise formulation reduces variability in results, making it a trusted solution across healthcare and academic institutions compared to generic or non-validated alternatives.

Typical User Profile and Application Scenarios
Used by microbiologists, lab technicians, and pathology staff in clinical, pharmaceutical, academic, and public health labs. Ideal for environments where diagnostic accuracy and reproducibility are essential for patient care, teaching, or microbial research.

Best Practices for Use and Maintenance

• Always wear PPE including gloves and eye protection during handling

• Store in a cool, dry place, out of direct sunlight

• Do not use past expiration date

• Clean staining surfaces promptly to avoid permanent dye stains

• Refer to SDS for spill and first aid measures

Regulatory and Safety Information
Labeled for laboratory use only. Not for internal or therapeutic use. Handle in accordance with laboratory chemical handling protocols. Contains dye and solvents that require careful handling as outlined in the SDS provided with the product.

How to Order
To order BD 212525 Gram Crystal Violet Reagent, add the product to your cart and complete your purchase through CIA Medicals secure online checkout. For bulk orders or product-related inquiries, please contact CIA Medical at (312) 275-5850 or use the contact form provided on this page.