BD 212542 - odine Gram Stain Stabilized Reagent, 250mL Bottle for Reliable Microbiological Staining
BD 212542 Iodine Gram Stain Stabilized is a laboratory-grade reagent used as the mordant step in the Gram staining process. Supplied in a 250 mL bottle, this stabilized iodine solution enhances crystal violet binding to bacterial cell walls, enabling accurate differentiation between Gram-positive and Gram-negative organisms. The formulation is optimized for consistency and shelf life, making it a dependable choice for high-throughput microbiology labs and clinical diagnostic environments.
| Manufacturer | Application | Container Type | Type | Volume |
| 212542 | Gram Iodine Stain | Bottle | Microbiology | 4 X 250 mL |
Key Benefits
Stabilized iodine formulation ensures consistent performance and extended usability
Enhances crystal violet adherence for clear Gram-positive staining
Helps ensure accurate and reproducible microbiological identification
Supplied in a convenient 250 mL bottle for routine lab workflows
Manufactured under strict quality standards to support diagnostic reliability
Compatibility and Applications
Compatible with BD and most standard Gram staining protocols and reagents. Commonly used in:
Clinical microbiology laboratories
Hospital and reference laboratories
Educational and research settings
Industrial and pharmaceutical microbiology labs
Usage Guidelines
Use as the mordant step after crystal violet application in the Gram stain procedure. Gently flood the prepared smear with iodine reagent and allow it to sit for the recommended time, typically 1 minute. Follow with a decolorizing agent and counterstain as per standard protocol. Store in a cool, dry location, away from direct light.
Certifications, Safety Standards, and Regulatory Approvals
Produced in compliance with CLSI-recommended practices for microbiological reagents. Each lot is tested for quality assurance. Packaged and labeled to meet U.S. regulatory and laboratory safety standards, including appropriate hazard communication labeling.
Comparison with Competitor Products
Compared to in-house or generic iodine solutions, BDs stabilized iodine reagent offers greater reliability, reduced lot-to-lot variability, and a longer shelf life. The formulation supports higher stain clarity and is less prone to degradation during storage.
Typical User Profile and Application Scenarios
Used by laboratory technicians, microbiologists, and diagnostic professionals performing Gram staining. Ideal in high-throughput lab environments requiring consistent, validated reagents for bacterial classification and identification.
Best Practices for Use and Maintenance
Store tightly closed at room temperature, protected from heat and light
Do not dilute unless specified by protocol
Use clean dropper or dispenser to avoid cross-contamination
Replace bottle cap immediately after dispensing
Follow all laboratory safety and handling procedures
Regulatory and Safety Information
The product is labeled with appropriate safety data including hazard identification per OSHA Hazard Communication Standards. Includes full SDS documentation for compliance with laboratory safety regulations.
How to Order
To order the BD 212542 Iodine Gram Stain Stabilized 250 mL reagent, add the item to your cart and proceed through the secure checkout process at CIA Medical. For bulk pricing or laboratory supply consultations, contact CIA Medical customer service at (312) 275-5850 or use the contact form on this page for immediate assistance.
