BD 221014 - Sabouraud Liquid Broth Enriched Medium for Cultivation of Fungi and Yeasts

BD 221014 - Sabouraud Liquid Broth Enriched Medium for Cultivation of Fungi and Yeasts

The BD 221014 Sabouraud Liquid Broth is a high-quality, ready-to-prepare microbiological medium formulated for the cultivation of fungi, yeasts, and molds. Developed by BD (Becton Dickinson), this broth is based on the classic Sabouraud formulation, originally designed by Raymond Sabouraud for dermatophyte culture. The mediums nutrient-rich compositioncontaining peptones and dextrosesupports robust fungal growth while its slightly acidic pH (around 5.6) inhibits bacterial contamination. BDs Sabouraud Liquid Broth ensures consistent performance in clinical, industrial, and research microbiology laboratories where accurate fungal cultivation and enumeration are essential.

Key Benefits

The BD Sabouraud Liquid Broth offers excellent growth characteristics for a broad range of fungal organisms, including dermatophytes, pathogenic yeasts such as Candida spp., and filamentous molds like Aspergillus spp. Its liquid formulation provides flexibility for quantitative culture work, fungal biomass production, and antifungal susceptibility testing. The products consistent batch quality, ease of preparation, and broad usability make it a reliable choice for laboratories seeking dependable fungal growth media. Additionally, its acidic nature minimizes bacterial interference, allowing for selective propagation of fungal species.

Compatibility and Applications

The BD 221014 Sabouraud Liquid Broth is compatible with a wide range of microbiological applications. It is commonly used in hospital and reference laboratories, pharmaceutical and cosmetic testing, food microbiology, and environmental monitoring. The broth is suitable for cultivating clinical isolates of Trichophyton, Microsporum, Epidermophyton, Aspergillus, and Candida species. It is also used in research settings for studying fungal metabolism, growth kinetics, and antifungal efficacy in liquid culture systems. The medium can be inoculated directly from swabs, scrapings, or fungal suspensions.

Usage Guidelines

To prepare the medium, suspend 30 g of BD Sabouraud Liquid Broth in 1 liter of purified water. Mix thoroughly and heat gently to dissolve the medium completely. Dispense into suitable containers and sterilize by autoclaving at 121C for 15 minutes. After sterilization, allow the medium to cool to room temperature before inoculation. Inoculate with the desired fungal specimen and incubate at 2530C. Observe for visible turbidity or fungal growth over 27 days, depending on the organism. Store dehydrated medium tightly sealed in a cool, dry place away from moisture and light.

Certifications, Safety Standards, and Regulatory Approvals

The BD 221014 Sabouraud Liquid Broth complies with ISO 11133 and ISO 13485 standards for microbiological media quality and manufacturing. It is produced under Good Manufacturing Practice (GMP) guidelines and meets FDA and CE requirements for in vitro diagnostic (IVD) media. Each batch undergoes rigorous quality control testing to ensure reproducibility, sterility, and pH stability, ensuring consistent laboratory performance across all applications.

Comparison with Competitor Products

Compared to other Sabouraud broths available on the market, BDs formulation provides superior clarity, consistency, and growth performance. Many alternative brands may exhibit variable pH or reduced fungal recovery rates, while BDs quality-controlled production ensures optimal nutrient balance and selectivity. Its consistent growth performance across multiple fungal species makes it a preferred choice for both diagnostic and industrial laboratories requiring reliable culture conditions.

Typical User Profile and Application Scenarios

This product is ideal for use by microbiologists, mycologists, and laboratory technologists in clinical and industrial settings. It is particularly suited for diagnostic laboratories performing fungal culture testing for dermatophytes and opportunistic fungi, as well as for research and pharmaceutical quality control teams conducting fungal growth and inhibition studies. It is also widely used in environmental testing laboratories monitoring fungal contamination in air, water, and materials.

Best Practices for Use and Maintenance

Use sterile technique throughout media preparation and inoculation to prevent contamination. Always verify the performance of each batch using known control organisms such as Candida albicans ATCC 10231 and Aspergillus niger ATCC 16404. Store the dehydrated medium in its original container with the lid tightly closed. Avoid overheating the medium during sterilization, as this may degrade the dextrose content. After inoculation, inspect for fungal growth daily and dispose of used cultures according to biosafety level 2 waste disposal standards.

Regulatory and Safety Information

The BD 221014 Sabouraud Liquid Broth is intended for professional laboratory use only. Personnel should wear appropriate personal protective equipment (PPE) such as gloves, lab coats, and eye protection when handling the medium and inoculated samples. Dispose of all used media and fungal cultures by autoclaving or incineration. Refer to the products Safety Data Sheet (SDS) for complete handling, hazard, and disposal instructions. BD ensures compliance with all applicable global safety and environmental regulations for laboratory culture media.

How to Order

To purchase the BD 221014 Sabouraud Liquid Broth, add the product to your cart and complete the secure checkout process on the CIA Medical website. For bulk or institutional orders, contact CIA Medical customer service at (312) 275-5850 or submit an inquiry through the contact form. CIA Medical provides prompt shipping, competitive pricing, and professional support for all BD microbiological and diagnostic culture media products.