BD 221026 - Simmons Citrate Agar Slant Dehydrated Differential Medium for Citrate Utilization Testing
The BD 221026 Simmons Citrate Agar Slant is a specialized microbiological medium formulated for the differentiation of enteric bacteria based on their ability to utilize citrate as a sole carbon source. This medium contains sodium citrate, ammonium salts, and bromothymol blue as a pH indicator, providing a reliable system for detecting citrate metabolism.
Prepared as a slanted agar, it offers a convenient surface for inoculation and observation. When organisms utilize citrate, alkaline byproducts are produced, resulting in a color change from green to blue. This clear visual indicator allows for accurate and efficient identification of citrate-positive organisms in clinical and research laboratories.
Key Benefits
The medium provides a simple and effective method for differentiating enteric organisms based on citrate utilization.
The distinct color change enhances readability and minimizes interpretation errors, supporting accurate diagnostic outcomes.
The slant format offers ease of use and efficient handling in routine laboratory workflows.
The dehydrated formulation ensures consistent performance, long shelf life, and reproducibility across batches.
Compatibility and Applications
BD Simmons Citrate Agar is compatible with standard microbiological equipment, including incubators and inoculating tools. It integrates seamlessly into routine diagnostic protocols and laboratory workflows.
This medium is widely used in hospitals, diagnostic laboratories, and research facilities for the identification and differentiation of Enterobacteriaceae and other Gram-negative organisms. It is commonly included in biochemical test panels for microbial identification.
Usage Guidelines
Prepare the medium according to the manufacturers instructions if supplied in dehydrated form, or use pre-prepared slants as provided. Inoculate the slant surface lightly using a sterile inoculating needle or loop.
Incubate under recommended conditions and observe for growth and color change. A transition from green to blue indicates a positive citrate utilization result.
Follow standard microbiological protocols and refer to the product insert for detailed preparation and incubation guidelines.
Certifications and Safety Standards
The BD 221026 Simmons Citrate Agar Slant is manufactured under ISO-certified quality systems, ensuring consistent quality, reliability, and performance.
Each batch is subjected to rigorous quality control testing to meet established microbiological standards, supporting its use in clinical and research environments.
Comparison with Competitors
Compared to non-standardized citrate media, BD Simmons Citrate Agar provides consistent composition and reliable indicator performance, ensuring accurate differentiation of organisms.
BDs strict manufacturing processes minimize variability between batches, whereas lower-quality alternatives may produce inconsistent results.
The trusted BD brand ensures dependable quality and performance, making this medium a preferred choice for laboratories requiring precise biochemical testing.
Typical User Profile and Application Scenarios
This product is intended for microbiologists, laboratory technicians, and clinical diagnosticians involved in bacterial identification and biochemical testing.
It is commonly used in hospital laboratories, diagnostic centers, research institutions, and public health facilities where accurate microbial differentiation is essential.
Best Practices for Use and Maintenance
Store the medium under recommended conditions to maintain its integrity and performance. Avoid contamination during handling and inoculation.
Use sterile techniques when inoculating the slant to ensure accurate results. Do not over-inoculate, as this may affect interpretation.
Monitor incubation conditions carefully and record observations promptly for reliable analysis.
Regulatory and Safety Information
The BD Simmons Citrate Agar Slant is intended for laboratory use and complies with applicable standards for microbiological media. It supports adherence to institutional and regulatory guidelines for safe handling and use.
Users must follow all relevant biosafety procedures when handling microbial cultures and dispose of used media in accordance with local regulations.
How to Order
To order the BD 221026 Simmons Citrate Agar Slant, add the product to your cart and proceed through CIA Medicals secure checkout process.
For bulk orders, institutional pricing, or additional assistance, contact CIA Medical customer service at (312) 275-5850. Their team is available to support your procurement needs.
