BD 221270 - BBL MacConkey II Agar Plate 100mm Culture Medium for Gram-Negative Bacteria Isolation, 100/BX
The BD 221270 BBL MacConkey II Agar Culture Plate is a high-quality, ready-to-use microbiological medium specifically formulated for the selective isolation and differentiation of gram-negative enteric bacteria. Each 100mm sterile petri dish is pre-filled with MacConkey II Agar, a refined version of the classic MacConkey formula enhanced for improved colony differentiation and consistent performance.
This culture medium is lactose-based and incorporates bile salts and crystal violet to inhibit gram-positive organisms, making it ideal for detecting coliforms and enteric pathogens in clinical, pharmaceutical, food, and water testing applications. The BD BBL plates are designed to deliver high performance in both manual and automated incubation systems used in modern laboratories.
Key Benefits:
Selective Isolation of Gram-Negative Bacteria: Inhibits gram-positive flora, allowing for clear, targeted recovery of organisms such as E. coli, Klebsiella, Salmonella, and Enterobacter.
Color Differentiation with Lactose Fermentation: Differentiates lactose fermenters (pink/red colonies) from non-fermenters (colorless colonies), simplifying preliminary identification.
Ready-to-Use and Sterile: Eliminates time-consuming preparation and sterilization, saving laboratory resources and improving workflow efficiency.
Consistent Lot-to-Lot Quality: Manufactured under stringent BD quality controls, ensuring reproducibility across multiple tests and applications.
Compatible with Standard Incubators: Designed for 3537C aerobic incubation protocols in clinical, research, and industrial labs.
Compatibility and Applications:
BD 221270 MacConkey II Agar plates are widely used in:
Clinical microbiology labs for identifying urinary tract and gastrointestinal infections
Pharmaceutical and biotech quality control labs
Food safety testing for pathogens in raw and processed food samples
Environmental monitoring of water sources for fecal contamination
Compatible with:
Manual streaking and colony isolation methods
Automated plate streaking and imaging systems
Standard laboratory incubators and storage equipment
Usage Guidelines:
Store plates in a refrigerator at 28C. Allow to come to room temperature before inoculation.
Using sterile technique, streak the specimen onto the agar surface.
Incubate aerobically at 3537C for 1824 hours or per specific laboratory protocols.
Observe colony morphology and color to preliminarily identify lactose-fermenting versus non-fermenting organisms.
Dispose of used plates in accordance with local biohazard waste regulations.
Always refer to your laboratorys standard operating procedures or BDs product insert for detailed instructions.
Certifications, Safety Standards, and Regulatory Approvals:
FDA Class I Medical Device (General Laboratory Use)
Manufactured in ISO 13485-Certified Facilities
CLSI-Recommended Culture Medium for gram-negative organism recovery
CE Marked for diagnostic use in Europe
Complies with USP / for microbial examination of non-sterile products (where applicable)
These standards ensure safe, effective, and traceable performance for clinical and industrial testing.
Comparison with Competitor Products:
Compared to MacConkey agar plates from Hardy Diagnostics or Thermo Scientific:
BDs BBL formulation offers enhanced selectivity and color differentiation, improving accuracy in differentiating coliforms.
Superior batch consistency ensures reliable results for regulated environments.
Durable packaging and stackable plate design reduce breakage and improve lab storage efficiency.
BD's established reputation in microbiology media makes it a preferred choice for institutions focused on compliance and quality.
Typical User Profile and Application Scenarios:
The BD 221270 MacConkey II Agar Plate is used by:
Clinical microbiologists in hospitals and diagnostic labs analyzing urine, feces, wound swabs, or respiratory samples
Lab technicians and quality control specialists in the pharmaceutical, food, and water industries
Research scientists investigating antibiotic resistance and microbial pathogenesis
Environmental health professionals monitoring bacterial contamination in public health settings
Typical scenarios include detection of enteric pathogens in stool samples, screening urine specimens for E. coli, or verifying the microbiological quality of pharmaceutical products.
Best Practices for Use and Maintenance:
Avoid freezing or exposing plates to direct sunlight or excessive moisture.
Do not use plates past the expiration date or if contamination or desiccation is visible.
Label and track batch numbers for quality control documentation.
Use proper aseptic technique to avoid false positives or cross-contamination.
Regularly validate media performance with known control strains such as E. coli ATCC 25922 and Salmonella enterica ATCC 14028.
Following these practices supports accurate microbial identification and reduces the likelihood of diagnostic errors.
Regulatory and Safety Information:
Labeled for in vitro diagnostic use (IVD)
Biohazard precautions applytreat all used plates as potentially infectious
Lot numbers and expiration dates are clearly printed on packaging for traceability
Backed by BD's Safety Data Sheets (SDS) and product technical documentation for regulated environments
These details assure end-users that the product adheres to high standards of quality, safety, and clinical accuracy.
How to Order:
To order the BD 221270 BBL MacConkey II Agar Plate 100mm:
Add the product to your cart and proceed through our secure checkout.
For bulk quantities, laboratory contracts, or specialized media inquiries, contact CIA Medical at (312) 275-5850 or submit a message using the contact form on this page.
Our product specialists are available to assist with compatibility questions, certifications, and logistics.
