BD 221508 - BBL Cooked Meat Medium Reliable Anaerobic Culture Medium for Clostridia and Other Anaerobes
The BD 221508 BBL Cooked Meat Medium is a high-quality, nutrient-rich culture medium formulated for the cultivation and maintenance of anaerobic bacteria, particularly Clostridium species and other obligate or facultative anaerobes. It consists of nutrient broth enriched with finely chopped, cooked meat particles that act as a reducing agent, supporting bacterial growth under oxygen-limited conditions. The medium promotes excellent recovery and long-term viability of anaerobes from clinical specimens, food, water, and environmental samples, making it a trusted standard in microbiology laboratories.
| Manufacturer | Application | Format |
| 221508 | Prepared Media | Tube Format |
Key Benefits
The BD 221508 Cooked Meat Medium provides optimal conditions for the growth, isolation, and preservation of anaerobic organisms. The meat particles supply amino acids and reducing agents, maintaining a low oxidation-reduction potential (Eh) necessary for anaerobic metabolism. The medium supports both vegetative growth and spore formation, ensuring robust recovery of Clostridium and other fastidious anaerobes. It is supplied in ready-to-use tubes for convenience, minimizing preparation time and reducing the risk of oxygen exposure or contamination.
Compatibility and Applications
The BD 221508 Cooked Meat Medium is compatible with a wide range of anaerobic and microaerophilic organisms, including Clostridium perfringens, Clostridium sporogenes, Peptostreptococcus, Bacteroides, and Fusobacterium species. It is used extensively in clinical, food, and industrial microbiology laboratories for:
- Isolation and cultivation of anaerobic bacteria from specimens and materials.
- Maintenance of stock cultures for long-term viability.
- Spore formation studies of Clostridium species.
- Testing bacterial proteolytic activity or gas production.
Usage Guidelines
Before use, visually inspect the tube to ensure the medium is free of contamination and excessive oxidation. Inoculate the medium aseptically with the specimen or bacterial culture using a sterile loop or pipette. Incubate at 3537C under anaerobic conditions for up to 48 hours or longer as required. Growth is indicated by turbidity, gas formation, or meat discoloration. For maintenance, subculture active cultures every few months onto fresh medium or store under refrigeration for short-term viability. Do not expose tubes to air for prolonged periods to maintain anaerobic integrity.
Certifications, Safety Standards, and Regulatory Approvals
The BD 221508 Cooked Meat Medium is manufactured under ISO 9001 and ISO 13485 certified quality management systems. It meets FDA and CE regulatory requirements for in vitro diagnostic (IVD) use and conforms to CLSI (Clinical and Laboratory Standards Institute) guidelines for anaerobic culture media. Each batch is validated for sterility, pH, and growth performance using control strains such as Clostridium sporogenes ATCC 11437 and Clostridium perfringens ATCC 13124.
Comparison with Competitor Products
Compared to other anaerobic culture media, BDs BBL Cooked Meat Medium offers superior reducing capacity, nutrient quality, and long-term stability. Many competitor brands experience premature oxidation or variability in meat particle composition, affecting anaerobic recovery rates. BDs consistent manufacturing process ensures uniform reduction potential, reproducible results, and extended shelf life, making it a gold standard for anaerobic bacterial culture and maintenance.
Typical User Profile and Application Scenarios
The BD 221508 Cooked Meat Medium is used by clinical microbiologists, laboratory technologists, food safety analysts, and research scientists who handle anaerobic bacterial cultures. It is ideal for hospital microbiology departments, public health laboratories, food industry quality control, and academic research environments investigating anaerobic pathogens or spoilage organisms. It is also used in clinical diagnostics for identifying Clostridium species from wound infections, gastrointestinal samples, and contaminated food products.
Best Practices for Use and Maintenance
Always handle the tubes aseptically and limit oxygen exposure by keeping the cap tightly closed. Do not overheat or autoclave sealed tubes, as pressure buildup may cause rupture. Store the medium at 225C, away from direct sunlight, and avoid freezing. For long-term culture maintenance, periodically transfer to fresh tubes or overlay with sterile mineral oil to preserve viability. Dispose of all used materials as biohazard waste following institutional and environmental safety protocols.
Regulatory and Safety Information
The BD 221508 Cooked Meat Medium is intended for professional laboratory use only. It is non-toxic but should be handled with appropriate biosafety precautions when used with infectious materials. Use gloves, lab coats, and eye protection while handling the medium and cultures. All work involving anaerobes should be conducted in an anaerobic chamber or jar under approved safety conditions. Follow local and institutional regulations for disposal of biological waste.
How to Order
To order the BD 221508 BBL Cooked Meat Medium, add the product to your cart and complete your purchase through CIA Medicals secure online ordering system. For institutional or bulk orders, contact CIA Medical customer service at (312) 275-5850 or use the contact form on this page. Our representatives can provide detailed product information, pricing, and documentation to support your laboratorys anaerobic culture and maintenance requirements.
