BD 221509 - BBL Motility Test Medium, K Tubes Differential Medium for Gram-Negative Bacilli Motility Detection, 10/BX
The BD 221509 BBL Motility Test Medium in K Tubes is a high-quality, ready-to-use differential medium designed for detecting motility in Gram-negative bacilli. Prepared in convenient test tube format, this semi-solid medium allows clear observation of organism movement away from the inoculation line. BDs precise formulation ensures consistent gel concentration, optimal clarity, and dependable performance when differentiating motile from non-motile bacteria in clinical and research microbiology settings.
| Manufacturer | 221509 |
| Application | Prepared Media |
| Format | Pour Tube Format |
| Storage Requirements | Requires Refrigeration |
| Type | Motility Test Medium |
Key Benefits
This motility medium provides reliable, easy-to-read results due to its standardized semi-solid consistency. The medium enables rapid differentiation of motile bacteriavisible as diffused growth throughout the tubefrom non-motile organisms that remain confined to the inoculation stab line. BDs controlled manufacturing ensures excellent reproducibility, stable pH, and uniform nutrient distribution, supporting accurate identification of enteric and non-enteric Gram-negative species.
Compatibility and Applications
BD BBL Motility Test Medium is compatible with standard inoculation procedures and is widely used for:
- Differentiation of motile Enterobacterales (e.g., Enterobacter, Escherichia, Proteus)
- Identification of non-motile organisms such as Klebsiella and Shigella
- Clinical diagnostic workflows for stool, urine, and sterile-site cultures
- Teaching microbiology students motility test interpretation
- Research applications evaluating bacterial movement and flagellar function
Usage Guidelines
Inoculate the medium by stabbing a sterile needle containing a pure colony straight into the center of the medium. Incubate at 3537C for 1824 hours. Motile organisms will show diffuse or hazy growth radiating from the stab line, while non-motile organisms will grow only along the inoculation track. Avoid disturbing the medium during handling to preserve interpretation accuracy. Store tubes at 28C in an upright position.
Certifications, Safety Standards, and Regulatory Approvals
BD BBL Motility Medium is produced under ISO 9001 and ISO 13485 certified quality systems and meets FDA and CE requirements for in vitro diagnostic (IVD) use. Each lot undergoes testing for sterility, gelling consistency, pH accuracy, and performance with reference motile and non-motile control strains.
Comparison with Competitor Products
Compared to generic motility media, BD tubes offer superior clarity, consistent semi-solid firmness, and more reliable differentiation between motile and non-motile organisms. Competitor products may suffer from uneven gelling or turbidity that complicates interpretation. BDs high-quality nutrient formulation ensures reproducible growth patterns and dependable diagnostic outcomes.
Typical User Profile and Application Scenarios
This medium is commonly used by:
- Clinical microbiology laboratories identifying enteric pathogens
- Hospital diagnostic labs evaluating Gram-negative infections
- Public health laboratories monitoring enteric organism profiles
- Academic institutions teaching differential media techniques
- Research labs studying microbial motility or virulence factors
Best Practices for Use and Maintenance
Use a straight inoculation stab to avoid false diffusion patterns. Avoid shaking or warming tubes excessively. Incubate tubes undisturbed and read within the recommended time window to prevent overgrowth or drying. Check tubes for cracking, dehydration, or contamination before use. Dispose of used cultures according to institutional biohazard protocols.
Regulatory and Safety Information
For in vitro diagnostic use only. Handle all cultures using biosafety level 2 precautions. Wear appropriate PPE and follow facility guidelines for transport, storage, and disposal of culture media and bacterial isolates.
How to Order
To order BD 221509 BBL Motility Test Medium K Tubes for Gram-Negative Bacilli, add the product to your cart and complete checkout through CIA Medicals secure ordering system. For bulk orders, laboratory contracts, or additional product support, contact CIA Medical customer service at (312) 275-5850 or submit an inquiry through the form on this page.
