BD 221669 - Nutrient Broth BBL General-Purpose Liquid Medium for Cultivation of Non-Fastidious Microorganisms, 10/BX

BD 221669 - Nutrient Broth BBL General-Purpose Liquid Medium for Cultivation of Non-Fastidious Microorganisms, 10/BX

The BD 221669 Nutrient Broth BBL is a high-quality, general-purpose liquid culture medium formulated for the growth, cultivation, and maintenance of a wide range of non-fastidious microorganisms. Made from peptones and beef extract, this broth provides essential nitrogen, amino acids, vitamins, and minerals required for robust microbial development. Its versatile formulation supports routine culture enrichment, organism propagation, and basic microbiological studies in clinical, industrial, and academic laboratories.

Key Benefits

BD 221669 Nutrient Broth offers excellent clarity, balanced nutrition, and reproducible performance, making it suitable for a wide variety of bacterial species. Its liquid format allows for uniform dispersion of organisms, consistent growth patterns, and improved recovery of stressed or low-count bacteria. The medium is manufactured under stringent BD quality standards, ensuring batch-to-batch consistency, reliable performance, and dependable results across all laboratory applications. Its simplicity and versatility make it ideal for general microbial cultivation, sterility testing, and foundational biological research.

Compatibility and Applications

This broth is compatible with a broad range of aerobic and facultative anaerobic bacterial species, including:

• Escherichia coli
• Bacillus subtilis
• Staphylococcus aureus
• Pseudomonas aeruginosa

Common applications include:

• Routine cultivation and maintenance of bacterial strains
• Microbial enumeration and dilution studies
• General research and experimentation
• Media preparation and enrichment before plating
• Quality-control testing in pharmaceutical, food, and water laboratories

Usage Guidelines

Rehydrate the dehydrated medium according to the manufacturers recommendations, typically by dissolving the specified amount in purified water. Mix thoroughly and heat gently until fully dissolved. Sterilize the broth by autoclaving at 121C for 15 minutes. Once cooled, dispense into sterile tubes or flasks as needed. Inoculate using aseptic technique and incubate at the appropriate temperature for your target organismcommonly 3537C for clinical isolates or variable temperatures for environmental species. Store the dehydrated medium in a cool, dry place.

Certifications, Safety Standards, and Regulatory Approvals

The BD 221669 Nutrient Broth BBL is produced in facilities certified under ISO 9001 and ISO 13485 quality systems. It meets FDA and CE regulations for in vitro diagnostic (IVD) use and undergoes extensive quality-control testing for sterility, pH accuracy, performance, and consistency. Each batch is validated using standard control organisms to ensure reliable growth and reproducibility.

Comparison with Competitor Products

Compared to competitor nutrient broths, BDs BBL Nutrient Broth offers enhanced clarity, superior nutritional consistency, and better batch reliability. Many lower-grade broths contain variable peptone quality or inconsistent mineral content, leading to erratic microbial growth. BDs high-quality formulation provides predictable results, making it a preferred choice for laboratories requiring dependable and reproducible outcomes.

Typical User Profile and Application Scenarios

The BD 221669 medium is widely used by microbiologists, laboratory technologists, researchers, and quality-control personnel. It is ideal for:

• Clinical diagnostic workflow support
• Academic teaching laboratories
• Industrial microbiology and environmental testing
• Research involving basic microbial physiology

Best Practices for Use and Maintenance

Prepare the medium using clean glassware and sterile technique. After sterilization, avoid prolonged heat exposure to preserve nutrient quality. Always label containers with preparation dates and batch numbers. Store reconstituted broth at 28C and use within recommended timeframes to maintain performance integrity. Dispose of inoculated media using approved biohazard protocols.

Regulatory and Safety Information

This product is intended for laboratory and in vitro diagnostic use by trained personnel. While nutrient broth itself is non-hazardous, cultures grown in it may contain pathogenic microorganisms requiring biosafety level 2 precautions. Use appropriate PPE including lab coats, gloves, and protective eyewear. Follow all local, institutional, and environmental regulations for handling and disposing of biological materials.

How to Order

To order the BD 221669 Nutrient Broth BBL, add the product to your cart and complete the purchase through CIA Medicals secure online checkout. For bulk quantities, laboratory contracts, or technical support, contact CIA Medical customer service at (312) 275-5850 or submit an inquiry using the contact form on this page. Our team is ready to assist with availability, pricing, and product specifications for your laboratorys needs.