BD 221787 - Thioglycollate Medium with Vitamin K Enriched Reducing Broth for Anaerobic and Fastidious Organism Recovery
The BD 221787 Thioglycollate Medium with Vitamin K is an enriched reducing broth formulated to support the cultivation, recovery, and maintenance of anaerobic, microaerophilic, and other fastidious microorganisms. This medium contains thioglycollate to reduce oxygen tension, along with vitamin K and additional growth factors that promote reliable recovery of delicate anaerobes during clinical, environmental, and research microbiology testing. BDs strict manufacturing processes ensure consistent performance, stable redox potential, and dependable organism viability.
Key Benefits
This thioglycollate medium provides a stable low-oxygen environment essential for preserving and cultivating strict anaerobes. The inclusion of vitamin K enhances the growth of organisms requiring fat-soluble vitamins, such as Bacteroides and other clinically significant anaerobic species. The mediums enriched composition improves organism recovery from specimens with low microbial load, while BDs high-quality preparation ensures uniformity, clarity, and reduced batch variation.
Compatibility and Applications
BD Thioglycollate with Vitamin K is compatible with standard anaerobic culture workflows and is widely used for:
- Primary cultivation of anaerobic bacteria from clinical specimens
- Transport and recovery of fastidious anaerobes
- Pre-enrichment before plating on anaerobic agar media
- Research involving anaerobic metabolic studies
- Routine diagnostic workups of wound, tissue, and sterile body site specimens
It supports a broad range of organisms including Clostridium, Bacteroides, Peptostreptococcus, and mixed anaerobic flora.
Usage Guidelines
Inoculate the medium using aseptic technique. Avoid excessive aeration while inserting specimens. Incubate at 3537C; allow anaerobic conditions to develop naturally within the reducing medium. For optimal performance, minimize repeated opening of the tube. Observe growth patterns, turbidity, and gas production for preliminary identification. Store unused media at 28C and protect from excessive light.
Certifications, Safety Standards, and Regulatory Approvals
BD Thioglycollate Medium with Vitamin K is produced in ISO 9001 and ISO 13485 certified facilities. It meets FDA and CE standards for in vitro diagnostic (IVD) use. Each lot is rigorously tested for sterility, pH accuracy, reducing potential, and expected organism recovery using certified control strains.
Comparison with Competitor Products
BD thioglycollate media offer enhanced reducing capacity and more consistent vitamin supplementation compared with generic preparations. Competing products may exhibit variable oxygen penetration or inconsistent nutrient availability, affecting anaerobe viability. BDs formulation ensures reliable growth and reproducibility, even for highly oxygen-sensitive organisms.
Typical User Profile and Application Scenarios
This medium is commonly used by:
- Clinical microbiology laboratories processing surgical, wound, or sterile-site cultures
- Hospital diagnostic units evaluating anaerobic infections
- Public health and reference laboratories studying anaerobic pathogens
- Research labs performing anaerobic physiology or metabolic assays
- Teaching laboratories demonstrating reducing media principles
Best Practices for Use and Maintenance
Avoid shaking or excessive mixing, as this reintroduces oxygen into the medium. Keep caps tightly closed to maintain reducing conditions. Rotate stock regularly to ensure media are used before expiration. If a surface oxidation layer forms, avoid inoculating directly into it. Dispose of used cultures according to institutional biohazard protocols.
Regulatory and Safety Information
For in vitro diagnostic use only. Handle all specimens under biosafety level 2 protocols. Wear appropriate PPE and follow facility guidelines for storage, handling, and disposal of clinical materials and culture media.
How to Order
To order BD 221787 Thioglycollate Medium with Vitamin K, add the product to your cart and complete checkout through CIA Medicals secure ordering system. For high-volume orders, laboratory supply agreements, or product support, contact CIA Medical customer service at (312) 275-5850 or submit an inquiry through the contact form on this page.
