BD 221815 - BBL Trypticase Soy Broth (TSB) 2 mL Sterile Ready-to-Use Culture Media for Microbial Growth, 100/Pk
The BD 221815 - BD BBL Trypticase Soy Broth (TSB) 2 mL is a sterile, ready-to-use liquid culture medium formulated to support the growth of a wide variety of fastidious and non-fastidious microorganisms. Manufactured by Becton Dickinson (BD), this high-quality broth is designed for clinical, industrial, and research laboratories that require reliable microbial cultivation in small volumes. The Trypticase Soy Broth medium provides a rich nutritional base containing enzymatic digests of casein and soymeal, offering the essential amino acids, nitrogen, and carbon required for robust microbial growth.
Each 2 mL tube of BD TSB is pre-sterilized and individually prepared for convenience in microbial testing, sterility checks, and quality control applications. It is ideal for laboratories requiring consistent results with minimal preparation time.
Key Benefits
BD Trypticase Soy Broth is a universal culture medium trusted for its performance, versatility, and reliability. Its primary benefits include:
- Broad spectrum growth support: Cultivates a wide range of bacteria, yeasts, and molds.
- Ready-to-use format: Eliminates the need for media preparation or sterilization, saving valuable lab time.
- High clarity: Transparent medium allows easy observation of microbial turbidity and growth patterns.
- Consistent results: Produced under strict quality control for reproducibility across tests.
- Versatile use: Suitable for sterility testing, microbial enumeration, and enrichment studies.
Compatibility and Applications
The BD 221815 Trypticase Soy Broth is compatible with standard laboratory workflows and microbial testing systems. Common applications include:
- Routine cultivation of aerobic and facultative anaerobic bacteria.
- Enrichment of microorganisms from clinical, food, and environmental samples.
- Sterility testing in pharmaceutical, cosmetic, and medical device manufacturing.
- Microbial recovery from environmental monitoring and quality assurance programs.
- Subculturing or maintenance of microbial isolates prior to identification or biochemical testing.
This medium is a staple in both diagnostic and industrial microbiology laboratories due to its versatility and reliability.
Usage Guidelines
Use aseptic technique when inoculating the BD 221815 Trypticase Soy Broth tube. Introduce the test organism using a sterile loop or pipette and incubate at the recommended temperature for the specific microorganism being cultured (typically 3537C for most bacteria). Observe for turbidity or other signs of growth after the appropriate incubation period. Do not reuse tubes or attempt to re-sterilize after use. Dispose of all used materials in accordance with biohazard waste protocols.
Certifications, Safety Standards, and Regulatory Approvals
The BD Trypticase Soy Broth is manufactured under ISO 13485:2016-certified quality management systems and complies with FDA, CE, and USP standards for microbiological culture media. It meets the specifications described in the United States Pharmacopeia (USP) and European Pharmacopeia (EP) for general-purpose growth media. Each batch undergoes strict quality control testing for sterility, pH (7.3 0.2 at 25C), and growth promotion using reference strains such as Staphylococcus aureus ATCC 6538, Escherichia coli ATCC 8739, and Pseudomonas aeruginosa ATCC 9027.
Comparison with Competitor Products
Compared to generic tryptic soy broths, BD BBL TSB provides superior lot-to-lot consistency and validated growth promotion for a broad spectrum of microorganisms. Competitor media may exhibit variable nutrient composition or inconsistent growth response. BDs controlled manufacturing process ensures optimal clarity, pH stability, and enhanced microbial recovery, making it a preferred choice for laboratories requiring reliable sterility testing and enrichment media.
Typical User Profile and Application Scenarios
This product is intended for use by microbiologists, laboratory technicians, and quality assurance professionals in:
- Clinical and hospital laboratories for routine microbial culture and sensitivity testing.
- Pharmaceutical and biotechnology companies performing sterility or bioburden testing.
- Environmental monitoring and food safety laboratories.
- Industrial and academic research institutions conducting microbial studies.
It is especially useful for laboratories that require standardized, ready-to-use tubes for fast, reliable microbial growth detection.
Best Practices for Use and Maintenance
Store BD Trypticase Soy Broth tubes at 225C in a dry environment, away from direct light. Do not freeze or overheat. Always inspect tubes before use for any turbidity, leakage, or discoloration, which may indicate contamination. Maintain aseptic technique during inoculation and handling to ensure reliable results. Discard any used or expired media following approved biohazard disposal protocols. For long-term culture maintenance, transfer growing organisms to fresh TSB or solid media as needed.
Regulatory and Safety Information
The BD 221815 - BD BBL Trypticase Soy Broth complies with global quality and safety regulations for in vitro diagnostic (IVD) use. It is non-toxic, non-pyrogenic, and free from animal-derived pathogens. A Safety Data Sheet (SDS) and Certificate of Analysis (CoA) are available from BD upon request. This medium is intended for professional laboratory use only and must be handled by trained personnel using standard microbiological practices.
How to Order
To order BD 221815 - BD BBL Trypticase Soy Broth 2 mL tubes, add the product to your cart and complete checkout through CIA Medical. We offer competitive pricing, fast delivery, and technical support for clinical, industrial, and research laboratories. For bulk purchases, Certificates of Conformance, or product performance data, contact CIA Medicals customer service team at (312) 275-5850 or use the contact form on this page. Our representatives are available to assist with availability, documentation, and technical inquiries.
