BD 228271 - Salmonella O Anti-Vi Antisera High-Quality Reagent for Serological Identification of Vi-Positive Salmonella Strains
The BD 228271 Salmonella O Anti-Vi Antisera is a high-specificity serological reagent designed for the accurate identification of Salmonella strains that express the Vi (virulence) capsular antigen. This antisera is widely used in microbiology laboratories to differentiate Salmonella enterica serovar Typhias well as other Vi-positive strainsfrom non-Vi strains through slide agglutination testing. The reagent is produced under BDs strict quality standards, ensuring reliable reactivity, strong agglutination patterns, and consistent diagnostic performance across routine and reference laboratory workflows.
| Manufacturer | Application | Form |
| 228271 | Microbiology Reagent | Lyophilized |
Key Benefits
BD 228271 Anti-Vi Antisera provides rapid, clear, and reliable agglutination reactions that support precise serotyping of Salmonella. The reagent is optimized for strong specificity, minimizing cross-reactivity and false positives. Its ready-to-use, sterile formulation reduces preparation time and ensures consistent performance across tests. By enabling accurate detection of Vi antigenexpressing isolates, the reagent helps guide appropriate clinical and epidemiological decision-making.
Compatibility and Applications
This antisera is compatible with standard slide agglutination procedures and a wide range of Salmonella identification algorithms. It is commonly used for:
- Identification of Salmonella Typhi (Vi-positive strains)
- Verification of Vi antigen presence in clinical isolates
- Epidemiological investigations and public health surveillance
- Reference laboratory serotyping workflows
BD 228271 is suitable for use in hospitals, diagnostic laboratories, food safety labs, and national reference centers involved in enteric pathogen monitoring.
Usage Guidelines
Perform slide agglutination using a clean glass slide. Emulsify a fresh bacterial colony in saline to form a smooth suspension. Add a drop of BD Anti-Vi Antisera and gently mix. Rock the slide for the recommended duration and observe for visible agglutination. Compare results with positive and negative controls. Avoid using cultures older than recommended, as Vi antigen expression may diminish. Store the reagent at 28C and do not freeze.
Certifications, Safety Standards, and Regulatory Approvals
The BD 228271 Salmonella O Anti-Vi Antisera is manufactured in ISO 9001 and ISO 13485 certified facilities. It complies with FDA and CE requirements for in vitro diagnostic (IVD) reagents. Each lot undergoes potency testing, specificity verification, sterility checks, and performance validation against known control strains to ensure consistent diagnostic accuracy and regulatory compliance.
Comparison with Competitor Products
BD antisera are recognized for their superior clarity, consistent reactivity, and minimal cross-reaction issues, making them more reliable than many competitor formulations. Some non-BD antisera may exhibit weaker agglutination, inconsistent titers, or variable shelf stability. BDs robust manufacturing and quality-assurance process ensure long-term reagent performance and dependable results across diverse testing environments.
Typical User Profile and Application Scenarios
This product is essential for microbiologists, clinical laboratory technologists, epidemiologists, and food safety professionals. It is particularly valuable in:
- Clinical diagnosis of typhoid fever
- Confirmation of Vi-positive Salmonella isolates
- Outbreak tracing involving enteric pathogens
- Public health serotyping and disease surveillance programs
Best Practices for Use and Maintenance
Use only fresh bacterial cultures to ensure strong and accurate antigen expression. Handle the reagent aseptically and avoid contamination of the dropper tip. Protect the antisera from heat and prolonged light exposure. Perform regular quality-control checks using known Salmonella Typhi positive controls. Discard any reagent that shows discoloration or precipitate formation inconsistent with normal appearance.
Regulatory and Safety Information
This antisera is intended for use by trained laboratory personnel. Wear appropriate PPE including gloves, lab coats, and eye protection during handling. Dispose of biological materials, used slides, and cultures as biohazard waste. Follow institutional biosafety level 2 practices when working with Salmonella isolates.
How to Order
To order the BD 228271 Salmonella O Anti-Vi Antisera, add the product to your cart and proceed through CIA Medicals secure online checkout. For bulk purchasing, laboratory procurement requests, or technical assistance, contact CIA Medical customer service at (312) 275-5850 or submit an inquiry via the contact form on this page. Our support team is ready to assist with availability, pricing, and product specifications for your serotyping needs.
