BD 231278 - Sensi-Disc Colistin CL-10 Disk Susceptibility Test for Polymyxin-Class Antibiotic Sensitivity
The BD 231278 Sensi-Disc Colistin CL-10 is a high-precision antimicrobial susceptibility testing (AST) disk containing 10 micrograms of colistin, a polymyxin-class antibiotic used primarily for treating multidrug-resistant Gram-negative bacterial infections. Each sterile disk delivers consistent antibiotic diffusion in disk diffusion assays such as the Kirby-Bauer method. Manufactured under BDs stringent quality standards, these Sensi-Discs ensure reproducible inhibition zone formation, enabling accurate detection of colistin susceptibility in clinically significant organisms.
Key Benefits
The BD 231278 Colistin CL-10 Sensi-Disc provides precise, reliable performance for AST programs targeting drug-resistant Gram-negative bacteria. The standardized 10mcg dosage ensures uniform diffusion from disk to agar, producing clearly defined zone edges for dependable interpretation. The sterile, single-use format reduces contamination risk and promotes workflow efficiency. With BDs robust manufacturing process, labs benefit from exceptional lot-to-lot consistency, making this disk an essential tool for identifying colistin susceptibility and supporting antimicrobial stewardship efforts.
Compatibility and Applications
The BD 231278 Sensi-Disc is compatible with standard Mueller-Hinton agar and follows CLSI and EUCAST recommendations for colistin disk diffusion testing when applicable. Although broth microdilution is the gold standard for colistin, disk diffusion may be used in research settings or preliminary screening. This disk is commonly used for evaluating organisms such as Acinetobacter baumannii, Pseudomonas aeruginosa, Klebsiella pneumoniae, and other carbapenem-resistant Enterobacterales (CRE). Applications include:
- Clinical microbiology laboratories
- Public health surveillance programs
- Pharmaceutical research involving polymyxin activity
- Antimicrobial resistance monitoring in high-risk environments
Usage Guidelines
Prepare a bacterial suspension equivalent to a 0.5 McFarland standard and inoculate the surface of Mueller-Hinton agar evenly. Using sterile forceps or a disk dispenser, place the BD 231278 Colistin 10mcg disk onto the agar surface. Incubate plates at 3537C for 1620 hours per institutional guidelines. Measure the inhibition zone diameter and compare values with the latest susceptibility interpretation standards. Store disks at 28C in a dry environment, and allow them to reach room temperature before opening the container to reduce condensation.
Certifications, Safety Standards, and Regulatory Approvals
The BD 231278 Colistin CL-10 Sensi-Disc is manufactured according to ISO 9001 and ISO 13485 certified quality systems. It complies with FDA and CE regulations for in vitro diagnostic (IVD) use. Each lot undergoes thorough testing for potency, zone reproducibility, diffusion uniformity, and sterility. BDs adherence to strict quality control ensures dependable AST performance across laboratory workflows.
Comparison with Competitor Products
Compared to generic susceptibility disks, BDs Colistin CL-10 Sensi-Discs provide superior potency retention, consistent diffusion patterns, and high manufacturing precision. Some competitor disks may suffer from inconsistent antibiotic loading or variable moisture resistance, causing unreliable results. BDs advanced impregnation and sealing technology ensures optimal antibiotic stability, clear zone edges, and dependable performance for screening polymyxin susceptibility.
Typical User Profile and Application Scenarios
The BD 231278 Sensi-Disc is used by microbiologists, laboratory technologists, infectious disease specialists, and researchers studying Gram-negative resistance mechanisms. It is particularly valuable in:
- Testing multidrug-resistant organisms in hospital settings
- Outbreak investigations involving CRE or CRAB
- Surveillance studies tracking colistin resistance
- Research on polymyxin mechanism of action and synergy testing
Best Practices for Use and Maintenance
Use sterile forceps when handling the disks and avoid touching the antibiotic surface. Keep disks stored with desiccants to maintain drug stability and prevent moisture absorption. Conduct quality control testing with recommended strains such as Escherichia coli ATCC 25922 and Pseudomonas aeruginosa ATCC 27853 to verify performance. Dispose of used disks and plates following biohazard regulations, and avoid using expired or compromised disks.
Regulatory and Safety Information
This product is intended for in vitro diagnostic use by trained laboratory personnel. Although the disk itself poses minimal chemical risk, procedures involving pathogenic bacteria require adherence to biosafety level 2 practices. Wear gloves, lab coats, and eye protection while performing susceptibility testing. Dispose of all materials used in testing in compliance with institutional and environmental safety guidelines.
How to Order
To order the BD 231278 Sensi-Disc Colistin CL-10, add the item to your cart and complete the checkout through CIA Medicals secure online ordering system. For bulk orders, lab-wide contracts, or technical questions, contact CIA Medical customer service at (312) 275-5850 or use the contact form on this page. Our team can provide product availability, pricing, and documentation to support your AST workflow.
