BD 231748 - BBL Taxo DrySlide Indole Reagent Rapid Indole Detection for Bacterial Identification, 75/PK
The BD 231748 BBL Taxo DrySlide Indole Reagent is a ready-to-use, high-performance diagnostic tool designed for the rapid detection of indole production by bacteria. Manufactured by BD (Becton Dickinson), this reagent is used to identify microorganisms capable of producing the enzyme tryptophanase, which converts tryptophan into indole. The reagent is embedded into a dry slide format, allowing direct and convenient testing without the need for liquid reagents. This dry formulation offers improved stability, portability, and ease of handling, making it ideal for clinical microbiology laboratories performing routine bacterial identification, especially within the Enterobacteriaceae family.
Key Benefits
The BD Taxo DrySlide Indole Reagent enables quick and reliable indole testing with minimal preparation. Its dry formulation eliminates the need for reagent mixing or additional solvents, reducing errors and simplifying workflow. Results are obtained in seconds, allowing for faster organism identification and improved laboratory efficiency. The reagent provides clear, easily interpretable color changes that indicate indole positivity. Its long shelf life and consistent performance make it a practical choice for both high- and low-volume laboratories seeking accuracy and convenience in bacterial differentiation tests.
Compatibility and Applications
The BD 231748 DrySlide Indole Reagent is compatible with bacterial cultures grown on standard nutrient or tryptophan-rich media, such as Trypticase Soy Agar (TSA) or Nutrient Agar. It is commonly used for the differentiation of indole-positive organisms such as Escherichia coli and Proteus vulgaris from indole-negative species like Klebsiella pneumoniae or Enterobacter cloacae. This reagent is applicable in hospital diagnostic laboratories, food safety testing, veterinary labs, and educational microbiology settings, where rapid bacterial identification is required.
Usage Guidelines
To perform the test, select a well-isolated colony from a 24-hour culture grown on an appropriate medium. Rub the colony gently onto the surface of the BD BBL Taxo DrySlide Indole reagent area using a sterile loop or wooden applicator stick. Observe for a color change within 1030 seconds. A blue-green to blue color indicates a positive indole reaction, while no color change or a light yellow color indicates a negative result. Avoid interpreting results after one minute, as delayed color changes may be misleading. Dispose of used slides according to institutional biosafety procedures.
Certifications, Safety Standards, and Regulatory Approvals
The BD 231748 BBL Taxo DrySlide Indole Reagent is manufactured under ISO 13485 and ISO 9001 certified quality systems and complies with FDA and CE regulations for in vitro diagnostic (IVD) devices. Each batch undergoes rigorous quality control testing using reference organisms, including Escherichia coli ATCC 25922 (positive control) and Enterobacter cloacae ATCC 13047 (negative control), ensuring consistent reagent sensitivity and specificity. BDs adherence to CLSI standards guarantees reproducible and accurate diagnostic results across clinical laboratories.
Comparison with Competitor Products
Unlike conventional liquid indole reagents, BDs DrySlide format provides a cleaner, safer, and faster testing method. Competitor liquid reagents may require additional solvents and can degrade quickly when exposed to light or air, resulting in inconsistent performance. The BD DrySlide Indole Reagent eliminates these issues through a stable dry chemical composition that maintains activity throughout its shelf life. Its portable, contamination-resistant design enables on-the-spot testing without additional equipment, reducing waste and handling errors. This superior stability and reliability make it a preferred choice in microbiology diagnostics worldwide.
Typical User Profile and Application Scenarios
This product is intended for use by microbiologists, laboratory technologists, and research professionals performing bacterial identification. It is commonly used in clinical microbiology labs for enteric pathogen identification, food safety and hygiene testing, and industrial microbiological quality control. The BD 231748 DrySlide Indole Reagent is especially beneficial in laboratories requiring rapid, point-of-use differentiation of indole-positive organisms in diagnostic workflows.
Best Practices for Use and Maintenance
Store the BD Taxo DrySlide Indole Reagent at 28C in its original, sealed container to preserve reactivity. Avoid exposure to direct sunlight, excessive humidity, or heat. Always use aseptic techniques to avoid cross-contamination when handling bacterial cultures. Do not touch the reagent surface directly with hands or metallic loops. Discard any used or contaminated slides immediately after testing. Check the expiration date before use and discard slides that show discoloration or other signs of deterioration.
Regulatory and Safety Information
This reagent is intended for professional use in laboratories only. Handle bacterial cultures under Biosafety Level 2 (BSL-2) precautions, following standard microbiological practices. Use appropriate personal protective equipment (PPE), including gloves, lab coats, and safety goggles. Avoid inhalation, ingestion, or direct contact with the reagent. In case of accidental exposure, rinse the affected area thoroughly with water. Refer to the Safety Data Sheet (SDS) for full handling and disposal instructions. BD guarantees compliance with international biosafety, environmental, and diagnostic quality standards.
How to Order
To purchase the BD 231748 BBL Taxo DrySlide Indole Reagent, add the product to your cart and complete the secure checkout process on the CIA Medical website. For institutional or bulk orders, contact CIA Medical customer service at (312) 275-5850 or use the online inquiry form. CIA Medical provides competitive pricing, reliable delivery, and professional support for all BD BBL and Difco microbiology reagents and identification products.
