BD 261198 - BBL Nitrate Reagent B Dropper 0.5mL Reliable Reagent for Bacterial Nitrate Reduction Testing, 50/BX
The BD 261198 BBL Nitrate Reagent B Dropper 0.5mL is a high-quality biochemical reagent formulated for the detection of nitrate reduction in microbiological identification procedures. Used as part of the standard nitrate reduction test, this reagent works in combination with Nitrate Reagent A to determine an organisms ability to reduce nitrate to nitrite or nitrogen gas. Each sterile, single-use dropper contains 0.5mL of pre-formulated Nitrate Reagent B, ready for immediate use in clinical and research laboratories. The convenient dropper format ensures accurate, contamination-free application and consistent color reactions for reliable results.
Key Benefits
The BD 261198 Nitrate Reagent B Dropper provides excellent precision, stability, and ease of use for routine bacterial differentiation tests. The single-use 0.5mL format minimizes waste and eliminates cross-contamination between samples. Its standardized concentration ensures consistent color intensity and reaction sensitivity across tests. Manufactured under BDs stringent quality control standards, this reagent provides reliable results, long shelf life, and optimal compatibility with BD BBL media and biochemical identification systems.
Compatibility and Applications
The BD 261198 Nitrate Reagent B is specifically designed for use with the Nitrate Reduction Test, a key biochemical assay in differentiating bacterial species, particularly within the Enterobacteriaceae family. It is used alongside Nitrate Reagent A (sulfanilic acid solution) to detect nitrite production after bacterial incubation in nitrate broth. The reagent is commonly used in clinical microbiology, research, and quality control laboratories for identifying organisms such as Escherichia coli, Klebsiella spp., and Pseudomonas aeruginosa. A red color formation after adding Reagents A and B indicates a positive nitrate reduction result.
Usage Guidelines
After incubating the bacterial culture in nitrate broth at 3537C for 2448 hours, add 5 drops of Nitrate Reagent A followed by 5 drops of BD 261198 Nitrate Reagent B to the culture tube. A red color appearing within 5 minutes indicates a positive result for nitrate reduction to nitrite. If no color develops, add a small amount of zinc dust to confirm complete nitrate reduction to nitrogen gas (a negative result will turn red after zinc addition). Store dropper vials at 28C, protect from light, and avoid freezing. Each dropper is intended for single use only.
Certifications, Safety Standards, and Regulatory Approvals
The BD 261198 Nitrate Reagent B Dropper is manufactured under ISO 13485 and ISO 9001 certified quality systems and complies with FDA and CE requirements for in vitro diagnostic (IVD) reagents. Each batch is tested for chemical accuracy, stability, and reaction performance in accordance with CLSI (Clinical and Laboratory Standards Institute) standards. BDs strict quality control ensures reagent uniformity and reproducibility across laboratories worldwide.
Comparison with Competitor Products
Compared to competitor biochemical reagents, BDs BBL Nitrate Reagent B Dropper provides superior consistency, convenience, and precision. Many competing products require manual pipetting or dilution, increasing the risk of error and contamination. BDs sealed single-use droppers eliminate preparation steps, ensuring aseptic handling and reproducible results. The reagents stable formulation and long shelf life reduce waste while maintaining high accuracy for routine diagnostic and research applications.
Typical User Profile and Application Scenarios
The BD 261198 Nitrate Reagent B Dropper is used by clinical microbiologists, laboratory technologists, and research scientists in hospital, pharmaceutical, and academic laboratories. It is essential for bacterial identification panels, biochemical profiling, and antimicrobial research. Typical applications include differentiating nitrate-reducing bacteria, validating bacterial metabolic capabilities, and confirming biochemical results during diagnostic identification workflows.
Best Practices for Use and Maintenance
Store BD Nitrate Reagent B Droppers at 28C and keep them sealed until use to prevent oxidation. Avoid direct light exposure, which can degrade the reagents activity. Use aseptic technique when adding to cultures. Do not reuse opened droppers; dispose of them immediately after use. For consistent performance, use in conjunction with BD Nitrate Reagent A Dropper (BD 261197). Handle all biological materials and reagents according to biosafety and chemical safety guidelines.
Regulatory and Safety Information
The BD 261198 Nitrate Reagent B Dropper is intended for in vitro diagnostic use only by trained laboratory personnel. It contains non-toxic components but should be handled with care. Avoid skin or eye contact; in case of exposure, rinse thoroughly with water. Always wear appropriate personal protective equipment (PPE) such as gloves, goggles, and lab coats. Dispose of used materials and cultures in compliance with institutional and local hazardous waste regulations.
How to Order
To order the BD 261198 BBL Nitrate Reagent B Dropper 0.5mL, add the product to your cart and complete your purchase through CIA Medicals secure online checkout system. For institutional or bulk orders, please contact CIA Medical customer service at (312) 275-5850 or use the contact form on this page. Our team can assist with product availability, pricing, and technical documentation for your laboratorys biochemical testing needs.
