BD 262710 - Middlebrook 7H10 Agar 500 g Dehydrated Medium for Mycobacteria Isolation and Cultivation
The BD 262710 Middlebrook 7H10 Agar 500 g is a specialized dehydrated microbiological medium formulated for the isolation and cultivation of Mycobacterium species, including organisms associated with tuberculosis. This medium provides a defined nutrient base that supports the growth of fastidious mycobacteria while allowing clear observation of colony morphology.
Middlebrook 7H10 Agar is enriched with essential nutrients, vitamins, and inorganic salts, creating an optimal environment for mycobacterial recovery. It is commonly supplemented with enrichment additives such as OADC to enhance growth performance. This medium is widely used in clinical microbiology laboratories, research institutions, and public health settings for reliable mycobacterial culture.
Key Benefits
Supports reliable growth and isolation of Mycobacterium species, including slow-growing organisms.
Defined formulation allows clear observation of colony morphology for accurate identification.
Compatible with enrichment supplements to enhance growth performance.
Dehydrated format ensures long shelf life, easy storage, and convenient preparation.
Compatibility and Applications
BD Middlebrook 7H10 Agar is compatible with standard microbiology laboratory equipment, including Petri dishes, incubators, and biosafety cabinets. It integrates seamlessly into mycobacterial culture workflows.
Common applications include tuberculosis diagnostics, mycobacterial isolation, antimicrobial susceptibility testing, and microbiological research. It is widely used in hospitals, diagnostic laboratories, public health institutions, and research facilities.
Usage Guidelines
Reconstitute the dehydrated medium using distilled or deionized water according to manufacturer instructions. Heat to dissolve completely and sterilize using standard autoclaving procedures.
Allow the medium to cool before adding any required supplements, then pour into sterile Petri dishes. Inoculate using aseptic techniques and incubate under appropriate conditions for mycobacterial growth.
Refer to the product insert and laboratory protocols for detailed preparation, supplementation, and incubation guidelines.
Certifications and Safety Standards
The BD 262710 Middlebrook 7H10 Agar is manufactured under ISO-certified quality systems, ensuring consistent performance, reliability, and high quality.
Each batch undergoes rigorous quality control testing to meet microbiological standards, supporting its use in professional laboratory environments.
Comparison with Competitors
Compared to egg-based media such as Lowenstein-Jensen, Middlebrook 7H10 Agar provides a more defined composition and improved visualization of colony morphology.
BDs stringent manufacturing processes ensure minimal batch variability, whereas lower-quality alternatives may compromise growth consistency and clarity.
The trusted BD brand ensures dependable performance, making this medium a preferred choice for laboratories specializing in mycobacterial culture.
Typical User Profile and Application Scenarios
This product is intended for microbiologists, laboratory technicians, and clinical diagnosticians involved in mycobacterial testing and research.
It is commonly used in hospital laboratories, tuberculosis testing centers, public health institutions, and research facilities where accurate detection and cultivation of Mycobacterium species are essential.
Best Practices for Use and Maintenance
Store the dehydrated medium in a cool, dry environment to prevent moisture absorption and maintain stability. Ensure containers are tightly sealed after use.
Prepare only the required quantity to maintain freshness and reduce waste. Use sterile techniques during preparation and inoculation to prevent contamination.
Monitor incubation conditions carefully, as mycobacteria require extended incubation periods for optimal growth.
Regulatory and Safety Information
The BD Middlebrook 7H10 Agar is intended for laboratory use and complies with applicable standards for microbiological media.
Users should follow all relevant biosafety procedures when handling mycobacterial cultures and dispose of used media in accordance with local regulations.
How to Order
To order the BD 262710 Middlebrook 7H10 Agar 500 g, add the product to your cart and proceed through CIA Medicals secure checkout process.
For bulk orders, institutional pricing, or additional assistance, contact CIA Medical customer service at (312) 275-5850. Their team is available to support your procurement needs.
