BD 274520 - Difco Phenylalanine Agar Medium 500 g Dehydrated Differential Medium for Phenylalanine Deamination Testing, EA
The BD 274520 Difco Phenylalanine Agar Medium is a specialized dehydrated microbiological medium designed for the detection of phenylalanine deaminase activity in bacteria. This differential medium enables the identification of organisms capable of deaminating phenylalanine to phenylpyruvic acid, a key biochemical characteristic used in microbial classification.
Commonly used in clinical microbiology laboratories, this medium plays an important role in differentiating members of the Enterobacteriaceae family, particularly organisms such as Proteus, Providencia, and Morganella species. The formulation ensures consistent performance, allowing for accurate and reproducible biochemical testing.
| Manufacturer | Application | Container Type | Type |
| 274520 | Culture Media | Bottle | Phenylalanine Agar |
Key Benefits
Provides a reliable method for detecting phenylalanine deaminase activity, supporting accurate bacterial identification.
The differential formulation enables clear and distinct results when used with ferric chloride reagent.
The dehydrated format ensures long shelf life, easy storage, and convenient preparation.
Consistent composition supports reproducibility across diagnostic and research applications.
Compatibility and Applications
BD Difco Phenylalanine Agar is compatible with standard laboratory equipment, including incubators, test tubes, and microbiological workstations. It integrates seamlessly into routine biochemical testing workflows.
Common applications include differentiation of Enterobacteriaceae, clinical diagnostics, and microbiological research. It is widely used in hospitals, diagnostic laboratories, public health institutions, and academic research facilities.
Usage Guidelines
Reconstitute the dehydrated medium using distilled or deionized water according to the manufacturers instructions. Sterilize by autoclaving and dispense into tubes to create agar slants.
Inoculate the surface of the slant using aseptic techniques and incubate under recommended conditions.
After incubation, add ferric chloride reagent to detect phenylpyruvic acid formation. A green color indicates a positive reaction.
Refer to the product insert and standard laboratory protocols for detailed preparation and interpretation guidelines.
Certifications and Safety Standards
The BD 274520 Difco Phenylalanine Agar Medium is manufactured under ISO-certified quality systems, ensuring consistent performance, reliability, and high quality.
Each batch undergoes rigorous quality control testing to meet microbiological standards, supporting its use in professional diagnostic and research environments.
Comparison with Competitors
Compared to non-standardized phenylalanine media, BD Difco Phenylalanine Agar offers superior consistency and reliability, ensuring accurate biochemical differentiation.
BDs stringent manufacturing processes minimize variability between batches, whereas lower-quality alternatives may produce inconsistent results.
The trusted BD Difco brand ensures dependable performance, making it a preferred choice for laboratories requiring precise biochemical testing media.
Typical User Profile and Application Scenarios
This product is intended for microbiologists, laboratory technicians, and clinical diagnosticians involved in bacterial identification and biochemical testing.
It is commonly used in hospital laboratories, diagnostic centers, public health institutions, and research facilities where accurate differentiation of bacterial species is essential.
Best Practices for Use and Maintenance
Store the dehydrated medium in a cool, dry environment to prevent moisture absorption and maintain product stability. Ensure the container is tightly sealed after use.
Prepare only the required quantity of medium to maintain freshness and reduce waste. Use sterile techniques during preparation and inoculation to prevent contamination.
Monitor incubation conditions carefully and use fresh reagents to ensure accurate interpretation of results.
Regulatory and Safety Information
The BD Difco Phenylalanine Agar Medium is intended for laboratory use and complies with applicable standards for microbiological media. It supports adherence to institutional and regulatory guidelines for safe handling and use.
Users should follow all relevant biosafety procedures when handling microbial cultures and dispose of used media in accordance with local regulations.
How to Order
To order the BD 274520 Difco Phenylalanine Agar Medium 500 g, add the product to your cart and proceed through CIA Medicals secure checkout process.
For bulk orders, institutional pricing, or additional assistance, contact CIA Medical customer service at (312) 275-5850. Their team is available to support your procurement needs.
