BD 298519 - MacConkey II Agar with Sorbitol Selective Differential Medium for E. coli O157 Detection
The BD 298519 MacConkey II Agar with Sorbitol is a selective and differential microbiological medium designed for the isolation and differentiation of Gram-negative enteric bacteria, with a specific focus on identifying Escherichia coli O157 strains. This formulation replaces lactose with sorbitol, enabling differentiation based on sorbitol fermentation.
Most E. coli strains ferment sorbitol, producing pink colonies, while E. coli O157 typically does not ferment sorbitol, resulting in colorless or pale colonies. This distinct differentiation makes the medium highly valuable for clinical diagnostics, food safety testing, and public health investigations.
| Manufacturer | 298519 |
| Application | Prepared Media |
| Format | Plate Format |
Key Benefits
Selective formulation inhibits Gram-positive organisms and supports Gram-negative enteric bacteria growth.
Differential properties allow clear identification based on sorbitol fermentation.
Facilitates rapid screening of E. coli O157 with distinct colony appearance.
Consistent formulation ensures reliable and reproducible results.
Compatibility and Applications
BD MacConkey II Agar with Sorbitol is compatible with standard laboratory equipment, including Petri dishes and incubators. It integrates seamlessly into routine microbiology workflows.
Common applications include detection of pathogenic E. coli O157 in clinical specimens, food products, and environmental samples. It is widely used in hospitals, diagnostic laboratories, food safety testing facilities, and public health institutions.
Usage Guidelines
Reconstitute the dehydrated medium using distilled or deionized water according to manufacturer instructions. Heat to dissolve completely and sterilize as recommended.
Pour into sterile Petri dishes and allow to solidify. Inoculate samples using aseptic techniques and incubate under appropriate conditions.
Observe colony color and morphology to differentiate sorbitol fermenters from non-fermenters.
Certifications and Safety Standards
The BD 298519 MacConkey II Agar with Sorbitol is manufactured under ISO-certified quality systems, ensuring consistent performance and reliability.
Each batch undergoes rigorous quality control testing to meet microbiological standards, supporting its use in professional laboratory environments.
Comparison with Competitors
Compared to standard MacConkey agar, this formulation provides enhanced differentiation for E. coli O157 detection.
BDs stringent manufacturing processes ensure consistent sorbitol concentration and indicator performance, whereas lower-quality alternatives may show variability.
The trusted BD brand ensures dependable results, making this medium a preferred choice for targeted pathogen detection.
Typical User Profile and Application Scenarios
This product is intended for microbiologists, laboratory technicians, and public health professionals involved in pathogen detection and food safety testing.
It is commonly used in clinical laboratories, food testing facilities, and research institutions where accurate identification of E. coli O157 is required.
Best Practices for Use and Maintenance
Store the dehydrated medium in a cool, dry environment to prevent moisture absorption and maintain stability.
Prepare only the required quantity to ensure freshness and reduce waste.
Use aseptic techniques during preparation and inoculation to prevent contamination.
Regulatory and Safety Information
The BD MacConkey II Agar with Sorbitol is intended for laboratory use and complies with applicable standards for microbiological media.
Users should follow all relevant biosafety procedures when handling microbial cultures and dispose of used media in accordance with local regulations.
How to Order
To order the BD 298519 MacConkey II Agar with Sorbitol, add the product to your cart and proceed through CIA Medicals secure checkout process.
For bulk orders, institutional pricing, or additional assistance, contact CIA Medical customer service at (312) 275-5850. Their team is available to support your procurement needs.
