BD 299107 - BBL Trypticase Soy Broth (TSB), 100 mL Culture Medium Bottle for Microbiological Growth and Sterility Testing, 10/BX
The BD 299107 - BD BBL Trypticase Soy Broth (TSB) is a 100 mL ready-to-use liquid culture medium designed for the cultivation of a wide variety of microorganisms, including aerobic and facultative anaerobic bacteria and fungi. Manufactured by Becton Dickinson (BD) under the BBL brand, this general-purpose nutrient-rich medium is widely used in clinical, industrial, and research microbiology laboratories for sterility testing, microbial enumeration, and growth promotion studies.
Formulated from casein and soybean meal digests, BD Trypticase Soy Broth supports robust microbial growth and serves as the standard base medium in sterility tests and antimicrobial susceptibility protocols. Each bottle is produced under strict quality controls to ensure consistency, sterility, and reproducible results.
| Manufacturer | Application | Container Type |
| 299107 | Prepared Media | Bottle |
Key Benefits
The BD BBL Trypticase Soy Broth (100 mL) offers several key advantages for laboratory applications:
- Broad-spectrum support: Promotes growth of a wide range of aerobic and facultative microorganisms, including fastidious species.
- Ready-to-use format: Supplied in pre-filled, sterile bottles to eliminate preparation time and reduce contamination risk.
- High-quality nutrient composition: Optimized formula ensures consistent growth and recovery of clinically significant organisms.
- Reliable sterility and lot consistency: Every batch undergoes rigorous QC testing for sterility, pH balance, and performance validation.
- Multi-application versatility: Suitable for sterility testing, enrichment cultures, and microbiological monitoring in pharmaceutical, clinical, and industrial labs.
Compatibility and Applications
The BD 299107 Trypticase Soy Broth is compatible with a variety of laboratory workflows and testing systems. It is commonly used in:
- Clinical microbiology for culturing blood, body fluids, and other specimens.
- Pharmaceutical and cosmetic sterility testing in quality control laboratories.
- Environmental monitoring and microbial detection in manufacturing areas.
- Food and beverage microbiological analysis for pathogen recovery.
- Growth promotion testing for media validation.
This medium can also be used as a base for antibiotic susceptibility testing or as an enrichment broth for subculturing organisms from selective media.
Usage Guidelines
Before inoculation, visually inspect the broth for clarity and bottle integrity. Using aseptic technique, inoculate the Trypticase Soy Broth with the desired sample or culture material. Incubate under appropriate temperature and atmospheric conditions depending on the organism type (commonly 3037C). Observe for visible turbidity, pellicle formation, or sediment, indicating microbial growth. Following incubation, use aseptic technique for any transfers or subcultures. Do not use bottles showing evidence of contamination or leaks before inoculation.
Certifications, Safety Standards, and Regulatory Approvals
The BD BBL Trypticase Soy Broth complies with FDA, USP, and EP (European Pharmacopoeia) standards for sterility testing media. It is manufactured under ISO 13485:2016 and ISO 9001-certified quality management systems. Each lot is tested for sterility, pH (7.3 0.2 at 25C), and growth performance using specified control strains such as Staphylococcus aureus, Bacillus subtilis, Escherichia coli, and Candida albicans. The product is CE-marked for in vitro diagnostic (IVD) use and validated for use in compliance with CLSI guidelines for microbiological media performance.
Comparison with Competitor Products
Compared to other nutrient broths, BDs Trypticase Soy Broth provides superior clarity, consistency, and growth recovery rates. While competitor media may show lot-to-lot variability or inconsistent pH balance, BDs stringent production standards ensure reproducible performance in critical sterility testing applications. The prefilled sterile format reduces preparation errors, contamination risks, and media waste, outperforming powder-based or manually mixed alternatives in efficiency and reliability.
Typical User Profile and Application Scenarios
This product is designed for laboratory professionals, microbiologists, and quality control analysts working in clinical, pharmaceutical, food, and industrial laboratories. Typical use scenarios include:
- Routine sterility and environmental monitoring in GMP-regulated facilities.
- Clinical culture enrichment for low-level bacterial recovery.
- Quality control testing of medical devices, injectables, and sterile pharmaceutical products.
- Growth validation and recovery efficiency testing for microbial strains in research laboratories.
The BD BBL Trypticase Soy Broth provides dependable performance in both regulatory and research environments.
Best Practices for Use and Maintenance
Store BD BBL Trypticase Soy Broth bottles at 225C in a dry, dark environment. Do not freeze or expose to direct sunlight. Avoid using media that appear cloudy, discolored, or show microbial growth before inoculation. Always handle under aseptic conditions to prevent contamination. After inoculation, seal bottles securely and label with sample information and incubation conditions. Dispose of used media following institutional biohazard protocols for infectious materials.
Regulatory and Safety Information
The BD 299107 - BD BBL Trypticase Soy Broth complies with FDA, CE, ISO, and USP standards for microbiological culture media. It is classified as a non-hazardous in vitro diagnostic reagent. The medium is non-toxic and contains no hazardous chemicals. A Safety Data Sheet (SDS) is available from BD upon request, providing detailed information on composition, handling, and disposal. The product is intended for professional laboratory use only and should be used by trained personnel familiar with microbiological techniques.
How to Order
To order the BD 299107 - BD BBL Trypticase Soy Broth (100 mL), add the item to your cart and complete checkout through CIA Medical. We offer competitive pricing, fast delivery, and full customer support for clinical, research, and industrial laboratories. For bulk orders, performance documentation, or technical assistance, contact CIA Medicals customer service team at (312) 275-5850 or use the contact form on this page. Our representatives are available to assist with product availability, validation certificates, and technical specifications.
