BD 299701 - Mycoflask DTM with Chloramphenicol, Dermatophyte Test Medium Slant, 10/BX
The BD 299701 - BD Mycoflask DTM (Dermatophyte Test Medium) with Chloramphenicol Slant is a selective culture medium formulated for the isolation and presumptive identification of dermatophytesthe fungi responsible for superficial skin, hair, and nail infections. Developed by Becton Dickinson (BD), this pre-poured slant medium incorporates chloramphenicol to inhibit bacterial contaminants and most saprophytic fungi, ensuring selective growth of dermatophytes such as Trichophyton, Microsporum, and Epidermophyton species. The Mycoflask format provides a convenient, ready-to-use, self-contained culture environment, ideal for both clinical and veterinary diagnostic applications.
The medium contains phenol red as a pH indicator, which produces a characteristic color change from yellow to red as dermatophytes metabolize proteins and release alkaline byproducts, providing a rapid and reliable presumptive identification system.
| Manufacturer | 299701 |
| Application | Prepared Media |
| Cultivation or Test Type | Detection and Presumptive Identification of Dermatophytes |
| Format | Slant Tube Format |
| Type | Dermatophyte Test Medium Modified with Chloramphenicol |
Key Benefits
The BD Mycoflask DTM with Chloramphenicol Slant provides accurate, selective isolation of pathogenic dermatophytes while minimizing interference from bacterial and environmental contaminants. The slant format offers an extended surface area for inoculation and optimal aeration for fungal growth. The inclusion of chloramphenicol enhances selectivity without compromising dermatophyte recovery. The distinctive color change reaction simplifies interpretation and reduces the need for complex laboratory procedures, making it highly efficient for routine mycological testing in clinical and reference laboratories.
Compatibility and Applications
The BD 299701 DTM Mycoflask is compatible with standard clinical and veterinary microbiology protocols and equipment. It is widely used for:
- Detection and identification of dermatophytes from skin, nail, and hair samples.
- Screening fungal infections in dermatology and veterinary practices.
- Teaching and training laboratories for demonstrating fungal culture techniques.
- Follow-up testing to monitor antifungal therapy efficacy.
The Mycoflask format allows easy inoculation and visual observation without the need for additional Petri dishes or specialized incubation systems.
Usage Guidelines
Before use, inspect the slant for sterility and color consistency. Using aseptic technique, inoculate the surface of the DTM slant with the specimen (e.g., skin scrapings, hair, or nail fragments). Loosely cap the flask to allow air exchange and incubate at 2530C for up to 14 days. Examine the medium daily for colony growth and color change. Dermatophyte colonies typically appear within 57 days and are accompanied by a red color shift in the medium. Non-dermatophyte organisms may grow without color change or with delayed discoloration. Confirm presumptive positive results with microscopic or biochemical tests before reporting.
Certifications, Safety Standards, and Regulatory Approvals
The BD Mycoflask DTM with Chloramphenicol is manufactured under ISO 13485:2016-certified quality management systems and complies with FDA and CE regulations for in vitro diagnostic (IVD) use. Each lot undergoes performance verification using control strains such as Trichophyton mentagrophytes and Candida albicans to ensure selectivity, sterility, and color stability. BD ensures that all media formulations adhere to CLSI and USP standards for culture media quality, consistency, and performance.
Comparison with Competitor Products
Compared to traditional DTM plates or non-selective fungal media, the BD Mycoflask offers enhanced convenience, safety, and performance. Its sealed, self-contained design minimizes contamination risks and reduces handling during incubation. The inclusion of chloramphenicol provides superior bacterial inhibition compared to competitor DTM media lacking antibiotics. The phenol red indicator in BDs formulation yields a faster and more distinct color transition, improving accuracy in differentiating dermatophytes from saprophytes. This combination of precision and ease-of-use makes BD Mycoflask a preferred choice in mycology diagnostics.
Typical User Profile and Application Scenarios
This medium is designed for use by microbiologists, dermatologists, veterinarians, and laboratory technologists involved in fungal diagnostics. Common application environments include hospital and clinical laboratories, veterinary clinics, dermatology offices, and public health facilities. It is particularly beneficial for rapid, point-of-care fungal testing and for practitioners who need a convenient, self-contained medium for direct specimen inoculation and interpretation.
Best Practices for Use and Maintenance
Store the BD Mycoflask DTM slants at 28C and avoid freezing. Allow the medium to reach room temperature before inoculation. Keep flasks tightly closed when not in use to maintain sterility and prevent dehydration. Do not use media that show signs of contamination, cracking, or dehydration. Always handle biological specimens using standard biosafety precautions and dispose of used flasks as biohazard waste after autoclaving. Regularly verify medium performance with known control strains to maintain quality assurance in diagnostic testing.
Regulatory and Safety Information
The BD 299701 - BD Mycoflask DTM with Chloramphenicol is intended for professional use in clinical or veterinary laboratories and complies with FDA and CE requirements for in vitro diagnostic (IVD) media. The medium contains phenol red indicator and chloramphenicol antibiotic; users should avoid ingestion and contact with skin or eyes. Wear appropriate personal protective equipment (PPE) such as gloves, lab coats, and eye protection during handling. Safety Data Sheets (SDS) are available from BD upon request for detailed information on composition, storage, and disposal procedures.
How to Order
To order the BD 299701 - BD Mycoflask DTM with Chloramphenicol Slant, add the product to your cart and complete the checkout process. CIA Medical provides fast delivery, competitive pricing, and dependable support for clinical and research laboratories. For bulk orders, performance documentation, or product-specific inquiries, contact CIA Medicals customer service at (312) 275-5850 or use the contact form on this page. Our team can assist with product availability, specifications, and technical support to meet your mycological testing needs.
