BD 362761 - CPT Sodium Citrate Tube 16mm x 125mm, 8.0mL Cell Preparation Tube for Plasma and Mononuclear Cell Separation, 60/CS
The BD 362761 CPT (Cell Preparation Tube) Sodium Citrate Tube is a specialized blood collection and processing device designed for the isolation of peripheral blood mononuclear cells (PBMCs) and plasma from whole blood. Manufactured by BD (Becton Dickinson), this 16mm x 125mm, 8.0mL tube integrates a sodium citrate anticoagulant with a polyester gel barrier and a density gradient liquid. This all-in-one design streamlines PBMC separation, reducing the need for manual layering and minimizing cross-contamination risks. Ideal for research and clinical applications, the BD CPT Sodium Citrate Tube ensures accurate, reproducible, and sterile separation of viable cells for immunology, hematology, and molecular studies.
Key Benefits
The BD 362761 CPT Sodium Citrate Tube offers unmatched convenience, precision, and reproducibility in cell isolation workflows. Its integrated gel barrier and density gradient medium provide consistent separation of mononuclear cells and plasma in a single centrifugation step, significantly reducing processing time. The prefilled sodium citrate anticoagulant preserves cellular integrity and prevents clotting without altering cell surface markers. The sterile, closed-tube system minimizes exposure to bloodborne pathogens and improves biosafety in the laboratory. BDs precision manufacturing ensures consistent gradient density and gel stability for high-purity PBMC recovery across samples.
Compatibility and Applications
The BD CPT Sodium Citrate Tube is compatible with standard clinical centrifuges capable of processing 16mm x 125mm tubes. It is commonly used in immunology, hematology, oncology, virology, and molecular biology research for isolating lymphocytes and monocytes from peripheral blood. Typical applications include flow cytometry, immunophenotyping, cytokine assays, vaccine response studies, and gene expression analysis. The tubes design allows plasma and PBMCs to be collected in a single, standardized workflow, ensuring consistency across research and diagnostic protocols.
Usage Guidelines
Collect blood directly into the BD 362761 CPT Sodium Citrate Tube using standard venipuncture technique. Immediately invert the tube gently 810 times to ensure proper mixing of the anticoagulant. Centrifuge according to BDs recommended protocoltypically at 1,800 RCF for 2030 minutes at room temperature. After centrifugation, distinct layers will form: plasma at the top, a mononuclear cell band above the polyester gel barrier, and red cells and granulocytes at the bottom. Carefully extract the PBMC layer using a sterile pipette. Store isolated cells under appropriate conditions for downstream processing or cryopreservation. Do not freeze the tube before centrifugation.
Certifications, Safety Standards, and Regulatory Approvals
The BD 362761 CPT Sodium Citrate Tube is manufactured under ISO 13485-certified quality systems and complies with FDA and CE regulations for in vitro diagnostic (IVD) use. It meets CLSI and GMP standards for blood collection and cell separation devices. Each tube is individually tested for sterility, anticoagulant concentration, gel performance, and barrier integrity. BD ensures consistency and reliability through rigorous lot-to-lot quality control and validation against reference cell separation criteria.
Comparison with Competitor Products
Compared to conventional density gradient methods (e.g., Ficoll-Paque), the BD CPT system eliminates multiple pipetting and layering steps, reducing hands-on time and variability. Competitor systems often require open-tube handling or manual reagent addition, increasing the risk of contamination and operator error. BDs integrated design ensures a standardized, closed, and safer workflow. The sodium citrate anticoagulant provides superior cell viability compared to heparin-based systems, minimizing platelet activation and maintaining PBMC morphology for downstream immunological assays.
Typical User Profile and Application Scenarios
This product is designed for clinical researchers, laboratory technologists, and biomedical scientists conducting immunological or hematological investigations. It is widely used in hospitals, academic research labs, biotechnology companies, and reference testing centers. The BD 362761 CPT Sodium Citrate Tube is particularly suited for studies requiring fresh, viable mononuclear cellssuch as immune response monitoring, biomarker discovery, and vaccine trialswhere standardization and sterility are critical.
Best Practices for Use and Maintenance
Store tubes at 425C in an upright position and protect them from direct sunlight. Do not use tubes beyond their expiration date or if the gel barrier is disrupted. Always follow BDs recommended centrifugation protocol to achieve optimal cell yield and purity. Handle tubes gently to prevent disruption of gradient layers post-centrifugation. Use aseptic techniques when extracting PBMCs to maintain sample integrity. Dispose of used tubes as biohazardous waste in accordance with institutional and regulatory safety guidelines.
Regulatory and Safety Information
This device is intended for professional use only. All blood specimens should be treated as potentially infectious and handled under Biosafety Level 2 (BSL-2) precautions. Wear appropriate personal protective equipment (PPE), including gloves, lab coats, and eye protection. Avoid excessive shaking or agitation of the tube to prevent hemolysis. Refer to the products Instructions for Use (IFU) and Safety Data Sheet (SDS) for detailed handling, centrifugation, and disposal instructions. BD ensures full compliance with global biosafety and environmental safety regulations.
How to Order
To purchase the BD 362761 CPT Sodium Citrate Tube (16mm x 125mm, 8.0mL), add the product to your cart and complete the secure checkout process on the CIA Medical website. For institutional or bulk orders, contact CIA Medical customer service at (312) 275-5850 or use the online inquiry form. CIA Medical offers competitive pricing, reliable shipping, and professional support for all BD blood collection and cell separation products.
