Health-O-Meter 4060 - Easicult COMBI(10 Tests/box)
Easicult Combi
Easicult Combi dip slides are used for rapid monitoring of microbial contamination in industrial environments such as paint manufacturing, cutting fluids, paper/pulp, and fuel tanks. The slide is covered on one side with TTC media which supports the growth of most common bacteria, and on the other side with Rose-Bengal media which supports the growth of fungi.
- Easy 3 step procedure; dip in fluid, incubate and read results
- Convenient and cost effective on-site testing
- Incubate at 27 to 30 degree celcius for 24-48 hours for detection bacteria; Yeast and molds need 3 days incubation at 27-30 degree celcius
- Helps prevent equipment breakdowns by detecting early signs of corrosion or contamination
- Ensures finished product quality
- Can be used as a transport media for samples
Easicult Combi Intended use
Easicult Combi slides are intended for monitoring of microbial contamination in various industrial environments. The test can be performed on-site, or the slides can be used as convenient transport media for samples.
The slide is covered on one side with TTC Agar (yellowish), which supports growth of most common bacteria, and on the other side with Rose-Bengal Agar (pink), which supports growth of fungi. The main significance of the test is that elevation of total microbial counts can be detected
Easicult CombiContents of the kit
Warnings and precautions of Easicult TTC
- Do not use the product beyond the expiry date marked on the kit.
- Do not touch the unused growth media.
- Store in upright position.
- Do not use the slide if you notice
- discoloration or dehydration of the growth medium
- detachment of the growth media from the plastic slide
- evidence of microbial growth.
- Because any growth on the Easicult Combi slide may be pathogenic, do not touch the used slide.
Easicult TTC Storage
Storage Store Easicult Combi at room temperature (1825 degree celcius / 6477 degree farhenhite) protected from draught, temperature fluctuations and light sources. Avoid storage near heat-generating appliances. Do not allow to freeze. The expiry date (year-month-date) is marked on the box and on the cap of each slide.
Easicult TTC Sampling and procedure (Fig. 15)
To avoid contamination, the growth medium should not come into contact with any other material than the one to be tested. On the other hand, it is important that the growth medium makes full contact with the material to be tested.
Interpretation of results (Fig. 6)
Cautiously remove the slide from its tube after incubation and determine the microbial count (number of colony forming units, CFU) by comparing the density of growth on the slide with the model chart. Note the separate model charts for TTC and RoseBengal. If the sample was diluted, the dilution factor must be taken into account in the evaluation. For example, if a dilution of 1 + 100 ml (1 ml of sample in 100 ml of water) shows a density of 106 CFU/ml, the actual result for the sample is 108 CFU/ml.
| CFU/ml | Contamination | |
104(< 104, 106, >106) | slight | usually no problems |
| moderate | ||
| heavy | not acceptable | |
Most aerobic bacteria grow on TTC Agar as red colonies. Moulds and yeasts may also grow slowly on this medium. Even though the bacterial growth is almost always in the form of red colonies, any colourless colonies should also be taken into account when the density is estimated. In cases where large colonies are present, it should be borne in mind that colony density, not the size of individual colonies, should be considered. If the bacterial count is very high (more than 107 CFU/ml), the growth is confluent. This can appear as a uniformly red surface layer. Very rarely there is totally colourless growth. It is advisable to compare slides exhibiting an apparently uniform surface with an unused slide to avoid misinterpretation
Easicult TTC Model Density Chart
