Lifesign 1002 - Uricult CLED-Polymyxin/EMB (10 Tests)
Uricult CLED-Polymyxin/EMB (Urinary Tract Infections)
10 Urine Culture-Paddles for the detection of bacteriuria and the presumptive identification of uropathogens. The test is intended for in vitro diagnostic use.
- Moderately Complex
- Culture method for urinary pathogens
- Easy to perform procedure; Dip - Incubate - Read Results
- Quick and convenient result within 24 hours
- Simple CLIA compliance for reporting colony counts.
Summary and explanation of the test
URICULT Urine CULTURE-PADDLES provide effective bacterial detection and presumptive identification in a simple and reliable manner. URICULT Urine CULTURE-PADDLES are attached to a screw cap. Each side of the culture-paddle is coated with an agar medium suitable for the growth of urinary bacteria, and the culture-paddle is suspended in a clear plastic vial.
The CULTURE-PADDLES are safely isolated in this vial during transport, incubation, storage and handling. Because of the uniform application of agar to the URICULT Urine CULTUREPADDLE, it is possible to obtain semi-quantitative results when the device is used as directed. This is determined by a simple visual comparison of bacterial growth on the agar surface with the Colony Density Chart provided. No actual colony counting is necessary.
Principles of the procedure
CLED-Polymyxin Agar (Cystine-Lactose-Electrolyte-Deficient/Polymyxin
CLED medium was first described by Mackey and Sandys1 specifically for use in dip inoculum procedures for urinary bacteriology. The electrolyte deficient nature of the medium prevents the characteristics swarming of Proteus. The inclusion of lactose and the indicator dye, Brom-Thymol Blue, allows differentiation of lactose fermenting bacteria, since the by-products of lactose fermentation cause the color of the agar to change from its original pale green color toward yellow. The incorporation of Polymyxin into the CLED medium creates a selective medium for the growth of Enterococci, Staphylococci, Proteus, and Yeast, preventing the growth of most E. coli, Klebsiella, and Enterobacter. Additionally, Pseudomonas colony growth is inhibited. Occasionally, mutant colonies of E. coli, Klebsiella, and Enterobacter will be observed on this medium as large mucoid colonies.
EMB Agar (Eosin Methylene Blue)
EMB agar is a selective reddish-brown colored agar recommended for the detection and isolation of Gram negative intestinal pathogenic bacteria. The balanced combination of the two dyes (Eosin and Methylene Blue) contained in the EMB agar makes it possible to distinguish lactose fermenting bacteria from nonfermenters. Those bacteria which ferment lactose will have dark colonies, purple or black in color. A green sheen may be observed across the agar surface during the growth of certain lactose fermenting bacteria
Reagents
| Uricult CLED/EMB | Product No. 1002 |
| Urine CULTURE-PADDLES (in individual vials) | 10 |
| Patient labels | 10 |
| Instructions for use containing the Colony Density Chart | 1 |
Typical formulation
| Uricult CLED-Polymyxin/EMB | EMB medium |
| Peptone 10.0 g/L | Peptone 10.0 g/L |
| Meat extract 3.0 g/L | Lactose 5.0 g/L |
| Lactose 10.0 g/L | Sucrose 5.0 g/L |
| L-Cystine 0.13 g/L | Dipotassium |
| Brom-Thymol Blue | Phosphate 2.0 g/L |
| Polymyxin | Eosin Y Methylene Blue |
Uricult CLED-Polymyxin/EMB Colony Density Chart
Procedure
Compliance with the following directions is required to achieve reliable test results.
