BD 212240 - Middlebrook OADC Enrichment, 100 mL Supplement for Mycobacterial Culture Media
The BD 212240 - BD Middlebrook OADC Enrichment (Oleic Acid, Albumin, Dextrose, and Catalase) is a sterile liquid supplement used to enrich Middlebrook 7H10 and 7H11 Agar, as well as 7H9 Broth, for the cultivation of Mycobacterium species. Manufactured by Becton Dickinson (BD) under the Difco and BBL brands, this 100 mL enrichment is essential for optimal growth and recovery of Mycobacterium tuberculosis and other slow-growing mycobacteria. The supplement provides critical nutrients, stabilizing agents, and protective enzymes that support bacterial metabolism and neutralize toxic compounds.
When combined with Middlebrook basal media, BD OADC creates a balanced, nutrient-rich environment that enhances colony formation and improves the reliability of mycobacterial culture results in both clinical and research laboratories.
Key Benefits
BD Middlebrook OADC Enrichment provides a precise nutrient formulation to promote optimal mycobacterial growth. Key benefits include:
- Essential nutrient enrichment: Supplies oleic acid, albumin, dextrose, and catalase required for the growth of fastidious mycobacteria.
- Protective function: Catalase neutralizes toxic peroxides, safeguarding bacterial cells during incubation.
- Enhanced growth performance: Improves colony morphology, pigment production, and recovery rates of Mycobacterium species.
- Consistent and reliable formulation: Manufactured under strict quality control to ensure batch-to-batch reproducibility.
- Convenient ready-to-use format: Sterile and pre-mixed, reducing preparation time and contamination risk.
Compatibility and Applications
The BD 212240 Middlebrook OADC Enrichment is specifically formulated for use with BD Middlebrook media. It is compatible with:
- Middlebrook 7H9 Broth for liquid culture and bacterial propagation.
- Middlebrook 7H10 Agar for isolation and enumeration of Mycobacterium species.
- Middlebrook 7H11 Agar for enhanced recovery of M. tuberculosis and other slow-growing strains.
Applications include clinical diagnostics, tuberculosis research, antimicrobial susceptibility testing, and mycobacterial preservation studies. It is widely used in hospital laboratories, public health institutions, and research facilities working with mycobacteria.
Usage Guidelines
To prepare enriched media, aseptically add 100 mL of BD Middlebrook OADC Enrichment to 900 mL of sterilized Middlebrook basal medium (7H9, 7H10, or 7H11). Mix thoroughly but avoid foaming. Dispense into sterile culture vessels or Petri plates under aseptic conditions. Do not autoclave after addition, as this may degrade heat-labile components. Incubate cultures at 3537C in appropriate CO2 conditions as required for Mycobacterium species. Follow biosafety level 3 (BSL-3) precautions when handling infectious materials such as M. tuberculosis.
Certifications, Safety Standards, and Regulatory Approvals
The BD Middlebrook OADC Enrichment is manufactured under ISO 13485:2016-certified quality systems and complies with FDA and CE regulations for in vitro diagnostic (IVD) products. Each batch is tested for sterility, pH (7.0 0.2), and growth promotion using standard reference strains, including Mycobacterium tuberculosis (H37Rv, ATCC 27294). The product meets ISO 11133 requirements for performance testing of microbiological culture media and is validated for use in clinical and research microbiology laboratories.
Comparison with Competitor Products
Compared to other enrichment formulations, BD Middlebrook OADC provides unmatched consistency, purity, and performance. Many generic OADC supplements may exhibit variability in albumin source or catalase activity, affecting mycobacterial growth and recovery. BDs proprietary process ensures optimal nutrient ratios and enzyme stability, resulting in superior growth rates and colony morphology. BD OADC also offers better long-term stability and reduced lot variation, making it the preferred choice in accredited diagnostic and research laboratories.
Typical User Profile and Application Scenarios
This product is intended for professional use by microbiologists, infectious disease researchers, and laboratory technologists in:
- Clinical laboratories performing tuberculosis culture and identification.
- Public health reference centers conducting drug susceptibility testing.
- Academic and research institutions studying Mycobacterium physiology and genetics.
- Pharmaceutical laboratories developing antimycobacterial compounds or vaccines.
The BD OADC Enrichment is essential for reliable isolation, maintenance, and propagation of mycobacteria in both liquid and solid culture formats.
Best Practices for Use and Maintenance
Store BD Middlebrook OADC Enrichment at 28C in its original container. Do not freeze. Ensure aseptic handling when adding to basal media to avoid contamination. Avoid repeated freeze-thaw cycles and prolonged exposure to light, which can degrade sensitive components such as catalase and dextrose. Discard any unused portion if turbidity, color change, or precipitate formation occurs. Always adhere to institutional biosafety protocols when working with pathogenic mycobacteria.
Regulatory and Safety Information
The BD 212240 - BD Middlebrook OADC Enrichment is CE-marked and FDA-cleared for laboratory use. It is non-toxic, non-pyrogenic, and manufactured from components of verified biological safety. The product contains bovine serum albumin (BSA) sourced from countries with negligible BSE risk, fully compliant with international animal-sourced material safety guidelines. A Safety Data Sheet (SDS) and Certificate of Analysis (CoA) are available upon request, providing detailed information on product handling, composition, and performance specifications.
How to Order
To order BD 212240 - BD Middlebrook OADC Enrichment (100 mL), add the product to your cart and complete checkout through CIA Medical. We offer competitive pricing, fast delivery, and technical support for clinical and research microbiology laboratories. For bulk orders, Certificates of Conformance, or additional documentation, contact CIA Medicals customer service team at (312) 275-5850 or use the contact form on this page. Our representatives are available to assist with product specifications, storage guidance, and batch certification inquiries.
