BD 212515 - TB Auramine O-Rhodamine Stain OD Fluorescent Stain for Acid-Fast Bacilli Detection
The BD 212515 TB Auramine O-Rhodamine Stain OD is a high-performance fluorescent staining reagent designed for the detection of acid-fast bacilli, including Mycobacterium tuberculosis, in clinical specimens. Manufactured by BD, a leader in microbiology and diagnostic solutions, this stain combines auramine O and rhodamine dyes to produce bright fluorescence under ultraviolet or LED fluorescence microscopy.
This staining method is widely used as a rapid screening tool in tuberculosis diagnostics. When applied to properly prepared smears, acid-fast organisms retain the fluorescent dye and appear as bright yellow-green or orange rods against a dark background. The OD formulation is optimized for consistent staining intensity and reliable performance, making it suitable for high-throughput laboratory environments.
The BD Auramine O-Rhodamine stain is an essential component in modern TB detection workflows, offering improved sensitivity compared to conventional staining methods.
Key Benefits
The BD 212515 stain enhances detection sensitivity, allowing laboratories to identify acid-fast bacilli more efficiently, even in samples with low bacterial load. The fluorescent signal improves visibility, reducing the time required for slide examination and increasing overall productivity.
Its ready-to-use formulation simplifies laboratory workflows, minimizing preparation steps and ensuring consistent results across tests. The stain produces clear and distinct fluorescence, supporting accurate interpretation and reducing the likelihood of false negatives.
By enabling faster and more reliable TB screening, this product contributes to timely diagnosis and improved patient outcomes.
Compatibility and Applications
The BD 212515 Auramine O-Rhodamine stain is compatible with standard fluorescence microscopy systems, including both ultraviolet and LED-based microscopes. It integrates seamlessly into laboratory workflows for mycobacterial detection.
Typical applications include clinical microbiology laboratories, hospital diagnostic centers, public health laboratories, and TB screening programs. It is widely used for examining sputum samples, tissue specimens, and other clinical materials suspected of containing acid-fast organisms.
Usage Guidelines
Ensure that slides are properly prepared and heat-fixed before staining. Apply the Auramine O-Rhodamine stain evenly to cover the smear and allow it to incubate for the recommended time.
Rinse gently with water and apply a decolorizer as specified in the staining protocol. After decolorization, apply a counterstain if required, then rinse and allow the slide to dry.
Examine the slide under a fluorescence microscope using appropriate filters. Acid-fast bacilli will appear as bright fluorescent rods against a dark background.
For detailed instructions, refer to the manufacturers protocol and laboratory guidelines.
Certifications and Safety Standards
The BD 212515 stain is manufactured under strict quality control systems that comply with internationally recognized standards such as ISO certification and Good Manufacturing Practices. These standards ensure consistent performance and reliability.
The product is intended for in vitro diagnostic use and aligns with regulatory requirements such as FDA clearance and CE marking where applicable. These certifications confirm that the reagent meets stringent safety and performance criteria.
Comparison with Competitor Products
Compared to traditional Ziehl-Neelsen staining methods, the BD Auramine O-Rhodamine stain offers significantly higher sensitivity and faster screening capabilities. While conventional stains require oil immersion microscopy, this fluorescent method allows rapid scanning at lower magnifications.
Compared to other fluorescent stains, BDs formulation provides consistent fluorescence intensity and reduced background interference, improving readability. Its reliability and ease of use make it a preferred choice over less standardized alternatives.
Typical User Profile and Application Scenarios
The BD 212515 stain is primarily used by microbiologists, laboratory technicians, and clinical diagnosticians involved in tuberculosis screening and mycobacterial research. It is also widely used in public health laboratories and TB control programs.
Common scenarios include routine screening of sputum samples, diagnostic evaluation of suspected TB cases, and large-scale screening initiatives in high-burden regions. Its efficiency makes it particularly valuable in high-volume laboratories.
Best Practices for Use and Maintenance
Store the stain according to manufacturer recommendations, typically in a cool, dark environment to preserve dye stability. Avoid prolonged exposure to light, which may degrade fluorescence intensity.
Ensure proper slide preparation and adherence to staining protocols for optimal results. Use clean reagents and avoid contamination during handling.
Regularly calibrate and maintain fluorescence microscopy equipment to ensure accurate visualization. Dispose of used reagents and materials according to laboratory safety guidelines.
Regulatory and Safety Information
The BD 212515 Auramine O-Rhodamine stain is intended for professional laboratory use only. Users should follow all biosafety protocols when handling clinical specimens and staining reagents.
Appropriate personal protective equipment should be used during handling. Compliance with regulatory standards ensures that the product meets required safety and performance benchmarks.
How to Order
Ordering the BD 212515 TB Auramine O-Rhodamine Stain OD is simple through CIA Medical. Locate the product on the website, add it to your cart, and proceed through the secure checkout process.
For bulk orders, pricing inquiries, or technical assistance, contact CIA Medical customer service at (312) 275-5850. Their team is available to support your laboratory diagnostic needs with expert guidance.
