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BD 212532 - Gram Safranin Stain OD Counterstain Reagent for Gram Staining Procedures
The BD 212532 Gram Safranin Stain OD is a high-quality microbiological staining reagent used as a counterstain in Gram staining procedures. This reagent is designed to provide clear contrast by staining Gram-negative organisms and background material, enabling accurate differentiation between Gram-positive and Gram-negative bacteria during microscopic examination.
Formulated for consistent and reliable performance, Safranin O is an essential component of Gram staining protocols widely used in clinical diagnostics, microbiology laboratories, and research settings. Its ready-to-use format ensures ease of application and supports standardized staining workflows.

Key Benefits
Provides clear and distinct contrast, allowing accurate differentiation of Gram-negative organisms.
Consistent staining quality ensures reliable and reproducible results across laboratory tests.
Ready-to-use formulation simplifies workflow and reduces preparation time.
Supports accurate microbial identification, improving diagnostic confidence.
Compatibility and Applications
BD Gram Safranin Stain OD is compatible with standard Gram staining procedures and integrates seamlessly into routine microbiological workflows.
Common applications include clinical diagnostics, bacterial identification, microbiological research, and educational laboratory use. It is widely used in hospitals, diagnostic laboratories, research institutions, and academic settings.
Usage Guidelines
Apply the safranin stain as the final step in the Gram staining procedure after decolorization. Allow the stain to remain on the slide for the recommended time before rinsing.
Examine the slide under a microscope to observe the staining results, where Gram-negative organisms appear pink to red.
Follow manufacturer instructions and laboratory protocols for proper staining techniques and timing.
Certifications and Safety Standards
The BD 212532 Gram Safranin Stain OD is manufactured under ISO-certified quality systems, ensuring consistent performance and reliability.
The product meets established standards for microbiological staining reagents, supporting safe and effective use in professional laboratory environments.
Comparison with Competitors
Compared to non-standardized staining reagents, BD Safranin O offers superior consistency and clarity, ensuring accurate Gram stain differentiation.
BDs stringent quality control processes minimize variability between batches, whereas lower-quality alternatives may produce inconsistent staining intensity.
The trusted BD brand ensures dependable performance, making it a preferred choice for microbiology laboratories.
Typical User Profile and Application Scenarios
This product is intended for microbiologists, laboratory technicians, and clinical diagnosticians involved in bacterial identification and staining procedures.
It is commonly used in hospital laboratories, diagnostic centers, research institutions, and educational laboratories where accurate Gram staining is essential.
Best Practices for Use and Maintenance
Store the reagent in a cool, dry environment away from direct light to maintain stability and performance.
Handle with care to avoid contamination and ensure accurate staining results. Use clean equipment and follow aseptic techniques.
Dispose of used materials in accordance with laboratory safety protocols and regulatory guidelines.
Regulatory and Safety Information
The BD Gram Safranin Stain OD is intended for laboratory use and complies with applicable standards for microbiological staining reagents.
Users should follow all relevant safety procedures when handling chemical reagents and dispose of waste according to local regulations.
How to Order
To order the BD 212532 Gram Safranin Stain OD, add the product to your cart and proceed through CIA Medicals secure checkout process.
For bulk orders, institutional pricing, or additional assistance, contact CIA Medical customer service at (312) 275-5850. Their team is available to support your procurement needs.
BD #212525, BD Gram Crystal Violet Reagent, Primary Stain for Gram Staining Procedures, 4/BX
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BD #212525, BD Gram Crystal Violet Reagent, Primary Stain for Gram Staining
$146.23 EACH
BD #371121, Gram Iodine Stain Reagent Mordant Solution for Gram Staining in Bacteriological Analysis,150/CS
$142.50 CASE
BD #212543, Gram Iodine Stain Reagent Mordant Solution for Gram Staining in Bacteriological Analysis
$507.97 EACH