BD 220830 - Trypticase Soy Agar (TSA) with Defibrinated Sheep Blood Enriched Medium for Isolation of Fastidious Organisms, 10/PK

BD 220830 - Trypticase Soy Agar (TSA) with Defibrinated Sheep Blood Enriched Medium for Isolation of Fastidious Organisms, 10/PK

The BD 220830 Trypticase Soy Agar (TSA) with Defibrinated Sheep Blood is an enriched, ready-to-use culture medium designed for the cultivation, isolation, and differentiation of a wide variety of bacteria, including fastidious species. Manufactured by BD (Becton Dickinson), this formulation combines nutrient-rich Trypticase Soy Agar with 5% defibrinated sheep blood, creating an ideal environment for the growth of both Gram-positive and Gram-negative organisms. The added sheep blood enables detection of hemolytic reactions, allowing for clear differentiation of -, a-, and ?-hemolytic bacteria commonly encountered in clinical microbiology. The BD 220830 medium provides reliable, consistent performance for routine diagnostic and research applications.

Key Benefits

BD TSA with Defibrinated Sheep Blood offers several advantages for laboratory professionals. Its enriched formulation supports robust growth of fastidious pathogens such as Streptococcus, Neisseria, and Haemophilus species. The medium allows easy observation of hemolytic patterns, critical for clinical identification and classification of bacterial isolates. Each batch is prepared with carefully standardized sheep blood to maintain consistent coloration, performance, and hemolytic clarity. The mediums balanced nutrient profile ensures high recovery rates, while BDs rigorous quality control process guarantees reproducibility and long shelf stability.

Compatibility and Applications

This medium is compatible with standard microbiological culture workflows and can be used in hospitals, diagnostic laboratories, research institutions, and public health agencies. It is commonly used for primary isolation of pathogenic bacteria from clinical specimens, including throat swabs, wound cultures, respiratory samples, and blood. BD TSA with Sheep Blood is also used for antibiotic susceptibility testing, colony morphology studies, and maintaining control strains. Its versatility makes it a core component in bacteriology and clinical diagnostics.

Usage Guidelines

Allow the BD TSA with Defibrinated Sheep Blood plates to reach room temperature before inoculation. Using aseptic technique, streak or inoculate the surface of the agar with the specimen or bacterial culture. Incubate the plates in an inverted position at 3537C for 1824 hours, or longer for slow-growing organisms. Observe colony morphology and hemolytic reactions: -hemolysis (complete clearing), a-hemolysis (greenish discoloration), or ?-hemolysis (no change). Dispose of used plates according to institutional biohazard waste protocols. Always follow standard laboratory safety practices and CLSI guidelines for culture interpretation.

Certifications, Safety Standards, and Regulatory Approvals

The BD 220830 TSA with Defibrinated Sheep Blood complies with ISO 11133 and ISO 13485 quality and performance standards for microbiological culture media. It is produced under Good Manufacturing Practices (GMP) and is CE-marked for in vitro diagnostic (IVD) use. BDs manufacturing facilities are FDA-registered, and every batch is tested with reference organisms such as Streptococcus pyogenes ATCC 19615 (-hemolysis), Streptococcus pneumoniae ATCC 6303 (a-hemolysis), and Enterococcus faecalis ATCC 29212 (?-hemolysis) to ensure consistent and reliable results.

Comparison with Competitor Products

Compared to similar blood agar media, BD 220830 is known for its superior consistency, clarity, and lot-to-lot uniformity. Competitor products may vary in blood coloration or hemolytic contrast, leading to interpretive uncertainty. BDs use of high-quality, standardized defibrinated sheep blood ensures optimal visibility of hemolytic zones and dependable growth performance. The products moisture balance and plate firmness also prevent media drying or surface cracking during incubation, ensuring reliable culture recovery and extended usability.

Typical User Profile and Application Scenarios

This product is widely used by microbiologists, laboratory technologists, and clinical professionals performing bacterial isolation and identification. It is particularly valuable in hospital microbiology labs for diagnosing respiratory infections, streptococcal diseases, and wound infections. Research institutions and pharmaceutical companies use it for microbial strain maintenance, antimicrobial testing, and quality control of bacterial cultures. The BD 220830 medium is also essential in veterinary diagnostics and environmental monitoring of bacterial contamination.

Best Practices for Use and Maintenance

Store the plates at 28C and protect them from light to preserve the quality of the blood component. Do not freeze or overheat the media. Allow plates to equilibrate to room temperature before inoculation to prevent moisture condensation. Handle using aseptic technique to avoid contamination. Examine plates regularly for contamination, desiccation, or discoloration before use. Dispose of expired or contaminated media according to biosafety level 2 (BSL-2) laboratory waste management standards.

Regulatory and Safety Information

The BD 220830 TSA with Defibrinated Sheep Blood is intended for professional laboratory use only. All microbial cultures should be handled as potentially infectious materials under appropriate biosafety procedures. Personnel should wear gloves, lab coats, and protective eyewear when handling specimens and cultures. After use, all contaminated materials must be autoclaved before disposal. Refer to the products Safety Data Sheet (SDS) for detailed information on handling, storage, and hazard management. BD ensures full compliance with international safety and performance standards for clinical diagnostic products.

How to Order

To purchase the BD 220830 Trypticase Soy Agar with Defibrinated Sheep Blood, add the item to your cart and proceed through the secure checkout on the CIA Medical website. For institutional or bulk orders, contact CIA Medical customer service at (312) 275-5850 or use the inquiry form for customized pricing and delivery options. CIA Medical provides fast shipping, competitive pricing, and dependable support for all BD microbiological culture media and diagnostic supplies.