BD 231569 - Trypticase Soy Agar (TSA) with Defibrinated Sheep Blood Enriched Medium for Isolation of Fastidious Organisms, 10/BX

BD 231569 - Trypticase Soy Agar (TSA) with Defibrinated Sheep Blood Enriched Medium for Isolation of Fastidious Organisms, 10/BX

The BD 231569 Trypticase Soy Agar (TSA) with Defibrinated Sheep Blood is a premium, enriched solid culture medium formulated for the cultivation and differentiation of fastidious and non-fastidious microorganisms. Manufactured by BD (Becton Dickinson), this medium combines nutrient-rich Trypticase Soy Agar with 5% defibrinated sheep blood to support the growth of a wide variety of bacterial species. It is specifically designed for isolating and identifying pathogens based on hemolytic properties, making it an essential tool in clinical, diagnostic, industrial, and research microbiology laboratories. The BD 231569 medium ensures consistent growth and distinct hemolytic pattern visualization, critical for accurate microbial identification.

Key Benefits

BD TSA with Defibrinated Sheep Blood provides superior nutritional support, enabling the recovery of fastidious organisms such as Streptococcus, Neisseria, and Haemophilus species. Its enriched formulation enhances bacterial recovery from clinical specimens while allowing differentiation of hemolysis types (-, a-, and ?-hemolysis). The use of standardized defibrinated sheep blood ensures clear and reliable hemolytic zones, minimizing variability between batches. Each plate offers optimal moisture balance and surface consistency, preventing cracking or drying during incubation. BDs stringent quality control processes guarantee reproducibility, sterility, and pH stability for dependable laboratory performance.

Compatibility and Applications

The BD 231569 TSA with Sheep Blood is compatible with standard bacteriological workflows and is widely used in hospitals, diagnostic laboratories, public health facilities, and research centers. It is particularly suited for the primary isolation and subculture of pathogenic bacteria from clinical specimens such as throat swabs, wound exudates, respiratory secretions, and body fluids. The medium is also utilized for antibiotic susceptibility testing, colony morphology observation, and maintaining control strains for quality assurance purposes. Its performance and reliability make it a cornerstone in clinical bacteriology and diagnostic microbiology.

Usage Guidelines

Before inoculation, allow the plates to reach room temperature to prevent moisture condensation. Using aseptic technique, streak the sample across the surface of the agar. Invert and incubate the plates at 3537C for 1824 hours under aerobic or microaerophilic conditions, depending on the organism. After incubation, examine colony morphology and hemolytic patterns-hemolysis (clear zone), a-hemolysis (greenish discoloration), and ?-hemolysis (no change). Dispose of used plates and materials following institutional biohazard protocols. For accurate results, handle all specimens and cultures using standard biosafety practices.

Certifications, Safety Standards, and Regulatory Approvals

The BD 231569 Trypticase Soy Agar with Defibrinated Sheep Blood complies with ISO 11133 and ISO 13485 quality management standards for microbiological media. It is manufactured under Good Manufacturing Practices (GMP) and is FDA-cleared and CE-marked for in vitro diagnostic (IVD) use. Each batch is tested for performance using quality control strains such as Streptococcus pyogenes ATCC 19615 (-hemolysis), Streptococcus pneumoniae ATCC 6303 (a-hemolysis), and Enterococcus faecalis ATCC 29212 (?-hemolysis), ensuring consistency, reliability, and diagnostic accuracy across all lots.

Comparison with Competitor Products

Compared to alternative blood agar formulations, BD 231569 offers unparalleled consistency, clarity, and growth performance. Competitor media may show inconsistent blood coloration or less distinct hemolysis, leading to misinterpretation. BDs standardized use of high-quality defibrinated sheep blood ensures reproducible color contrast and reliable hemolytic differentiation. The mediums robust agar matrix prevents excessive surface moisture and supports uniform bacterial colony distribution. BDs reputation for precision-engineered media makes this product a preferred choice among clinical laboratories requiring dependable diagnostic results.

Typical User Profile and Application Scenarios

This product is widely used by clinical microbiologists, laboratory technologists, and research scientists in medical and veterinary diagnostic laboratories. It is essential for identifying pathogenic Streptococcus and Staphylococcus species and for culturing fastidious organisms from human or animal samples. The medium also serves as a standard in academic microbiology teaching laboratories for demonstrating bacterial growth characteristics and hemolytic reactions. In industrial microbiology, it is used for contamination monitoring and microbial quality assurance testing.

Best Practices for Use and Maintenance

Store plates at 28C in their original packaging to prevent dehydration and protect from light. Do not freeze or expose to excessive heat. Use aseptic technique during inoculation to avoid contamination. Examine plates before use for any signs of contamination, desiccation, or color change. For best results, incubate promptly after inoculation and interpret results within recommended timeframes. Dispose of used cultures and materials in compliance with biosafety level 2 (BSL-2) laboratory waste procedures. Always verify media performance using appropriate control strains.

Regulatory and Safety Information

The BD 231569 TSA with Defibrinated Sheep Blood is intended for professional laboratory use only. All microbial cultures should be handled as potentially infectious materials following universal