BD 221020 - BBL Selenite-F Broth Tubes 8mL Selective Enrichment Medium for Isolation of Salmonella, 10/PK
The BD 221020 BBL Medium Culture Selective Selenite-F Broth Tubes (8mL) are ready-to-use selective enrichment media designed for the isolation of Salmonella species from clinical and food samples. Manufactured by BD (Becton Dickinson), this medium provides an optimized environment for the recovery of Salmonella while inhibiting the growth of competing intestinal flora such as coliforms and fecal streptococci. Each prefilled 8mL tube contains Selenite-F Broth formulated according to Leifsons modification, ensuring consistent performance and superior recovery rates in stool, urine, and other biological sample analyses.
Key Benefits
The BD BBL Selenite-F Broth Tubes offer convenience, accuracy, and reliability in detecting Salmonella from mixed microbial populations. The prefilled, sterile tubes reduce preparation time and minimize contamination risk. Selenite and phosphate buffer components selectively inhibit Gram-positive and many Gram-negative organisms, enhancing the growth of Salmonella. The ready-to-use format eliminates the need for media preparation, ensuring consistent results and compliance with quality assurance standards. BDs high manufacturing precision guarantees reproducibility and superior recovery of enteric pathogens from clinical and food samples.
Compatibility and Applications
The BD 221020 Selenite-F Broth is compatible with standard bacteriological techniques and downstream subculturing on selective agars such as XLD (Xylose Lysine Deoxycholate), HE (Hektoen Enteric), or SS (Salmonella-Shigella) agar. It is widely used in clinical microbiology laboratories for isolating Salmonella from stool specimens, and in food and water microbiology testing for pathogen screening. This selective broth supports the enrichment phase in both diagnostic and surveillance workflows for enteric infections and foodborne outbreaks.
Usage Guidelines
Inoculate an 8mL BD BBL Selenite-F Broth Tube with 12 drops of the test specimen (e.g., stool suspension or enrichment culture) using aseptic technique. Incubate the tube at 3537C for 1824 hours under aerobic conditions. After incubation, subculture a loopful from the broth onto selective agar plates such as XLD or HE agar. Incubate the plates at 3537C and examine for characteristic Salmonella colony morphology. Avoid prolonged incubation beyond 24 hours, as the inhibitory effect of selenite decreases, potentially allowing overgrowth by competing organisms. Dispose of all cultures and materials according to biosafety regulations.
Certifications, Safety Standards, and Regulatory Approvals
The BD 221020 Selenite-F Broth Tubes are manufactured under ISO 13485 and ISO 9001-certified quality systems. They meet FDA and CE regulatory standards for in vitro diagnostic (IVD) products. The product is produced in compliance with Good Manufacturing Practices (GMP) and validated using reference strains including Salmonella enterica ATCC 14028 to confirm performance. BDs stringent quality control ensures consistent formulation, pH balance, sterility, and selective performance in every lot.
Comparison with Competitor Products
Compared to conventional or manually prepared Selenite-F broths, BDs prefilled 8mL tubes provide unmatched convenience, consistency, and reliability. Competitor media may exhibit pH variation or uneven inhibition of non-target flora, affecting recovery rates. BDs Selenite-F Broth formulation ensures optimized selectivity and enrichment conditions for Salmonella while maintaining high recovery rates for low-level contamination samples. The prefilled, sealed tubes reduce contamination risks and ensure uniform sample processing across multiple tests.
Typical User Profile and Application Scenarios
This product is designed for clinical microbiologists, food safety analysts, and research scientists performing pathogen screening and enteric disease surveillance. It is ideal for hospitals, public health laboratories, food industry quality control facilities, and academic research institutions. The BD 221020 Selenite-F Broth Tubes are particularly useful for detecting Salmonella in stool cultures during suspected gastroenteritis outbreaks or foodborne infection investigations.
Best Practices for Use and Maintenance
Store the tubes at 28C and protect them from light and freezing. Use aseptic technique during inoculation to prevent cross-contamination. Do not reheat or reuse the medium. Always label tubes clearly with sample information and incubation details. Verify medium performance regularly using control organisms to ensure reliability. Avoid exposure to heat or prolonged storage, which can degrade the selective properties of the broth. Dispose of used materials in compliance with institutional biosafety protocols.
Regulatory and Safety Information
This product is intended for professional laboratory use only. Handle all biological specimens as potentially infectious materials under Biosafety Level 2 (BSL-2) conditions. Use appropriate personal protective equipment (PPE), including gloves, lab coats, and eye protection. Selenite compounds are toxic; avoid ingestion, inhalation, or contact with skin and eyes. Dispose of all contaminated materials by autoclaving before disposal. Refer to the products Safety Data Sheet (SDS) for detailed hazard and safety information. BD ensures full compliance with global safety and environmental regulations for diagnostic laboratory products.
How to Order
To purchase the BD 221020 BBL Selenite-F Broth Tubes 8mL, add the product to your cart and proceed through the secure checkout process on the CIA Medical website. For institutional or bulk orders, contact CIA Medical customer service at (312) 275-5850 or submit an inquiry via the online form. CIA Medical offers competitive pricing, rapid delivery, and professional support for all BD BBL culture media and microbiology diagnostic products.
