BD 221021 - BBL Selenite-F Broth, 8mL Tubes Enrichment Medium for Isolation of Salmonella Species, 100/BX
The BD 221021 BBL Medium Culture Size K Tubes containing 8mL of Selenite-F Broth are designed as an enrichment medium for the selective isolation of Salmonella species from clinical, food, and environmental samples. Manufactured by BD (Becton Dickinson), this pre-filled tube format offers a convenient, ready-to-use solution that supports the recovery of Salmonella while inhibiting the growth of competing intestinal flora such as coliforms. The balanced formulation of peptone, lactose, sodium selenite, and phosphate buffer ensures optimal enrichment conditions for low levels of Salmonella organisms in complex specimens. Each tube contains 8mL of sterile medium, prepared under controlled conditions for consistent and reliable performance.
Key Benefits
BD Selenite-F Broth Tubes provide a rapid and efficient method for enriching Salmonella from mixed microbial populations, especially in stool or food samples. The ready-to-use tubes eliminate the need for in-house media preparation, saving time and ensuring reproducibility. Sodium selenite selectively inhibits Gram-positive bacteria and certain Gram-negative competitors, allowing the growth of Salmonella and Shigella. The formulations optimized pH and nutrient balance enhance the recovery rate of target pathogens. BDs stringent manufacturing quality guarantees consistent selectivity and sensitivity in each batch, meeting clinical and food microbiology standards for pathogen detection.
Compatibility and Applications
The BD 221021 Selenite-F Broth Tubes are compatible with standard microbiological workflows and are typically used as an enrichment step prior to subculture on selective plating media such as XLD Agar, HE Agar, or Brilliant Green Agar. The medium is suitable for clinical specimens (e.g., feces), food samples, water testing, and environmental monitoring. It is routinely employed in public health, clinical, and food safety laboratories for isolating Salmonella in diagnostic and surveillance studies. The pre-filled 8mL tubes are ideal for laboratories that require sterile, convenient, and high-performance enrichment media for routine pathogen screening.
Usage Guidelines
Inoculate the BD Selenite-F Broth Tube (8mL) with approximately 0.51g of fecal specimen or 1mL of a pre-enrichment broth culture. Incubate the tube at 3537C for 1218 hours without agitation. After incubation, subculture the broth onto selective media such as XLD or HE agar for isolation and identification of Salmonella. Do not autoclave or reheat the medium, as heat may deactivate the selective components. Sodium selenite is toxichandle with care and avoid prolonged incubation, which may inhibit target organism recovery. Always include appropriate positive and negative control cultures to verify enrichment performance.
Certifications, Safety Standards, and Regulatory Approvals
The BD 221021 BBL Selenite-F Broth Tubes comply with ISO 13485 and ISO 9001 standards for quality management and are manufactured under Good Manufacturing Practices (GMP). The product meets FDA and CE requirements for in vitro diagnostic (IVD) use and is validated according to CLSI and ISO 11133 microbiological performance testing standards. Each lot is quality controlled using reference organisms such as Salmonella enterica serovar Typhimurium ATCC 14028 (positive control) and Escherichia coli ATCC 25922 (negative control) to ensure selectivity, sterility, and enrichment efficiency.
Comparison with Competitor Products
Compared to conventional or in-house prepared Selenite broths, BD Selenite-F Broth Tubes offer superior consistency, sterility, and selectivity. Competitor products may exhibit variable sodium selenite concentrations or inconsistent pH, leading to reduced target recovery or false negatives. BDs advanced manufacturing process ensures precise formulation and pre-measured volumes for optimal performance. The ready-to-use tubes also eliminate media preparation time, reducing labor costs and contamination risks. BDs long-standing reputation in microbiological diagnostics ensures dependable results across a wide range of specimen types and testing conditions.
Typical User Profile and Application Scenarios
This product is intended for use by trained laboratory professionals, including clinical microbiologists, food safety analysts, and environmental health technicians. It is ideal for hospital laboratories performing stool culture screening, food and beverage companies conducting pathogen surveillance, and public health agencies monitoring enteric infections. The BD 221021 Selenite-F Broth Tubes are particularly suited for detecting Salmonella in specimens with low bacterial loads, providing a critical enrichment step before confirmatory culture or molecular analysis.
Best Practices for Use and Maintenance
Store the tubes at 225C in a dry, dark environment. Do not freeze or expose to direct sunlight. Inspect tubes before use for any signs of contamination, color change, or drying. Use aseptic techniques when inoculating and handling the medium. After use, dispose of all materials in compliance with institutional biosafety and hazardous waste protocols due to the presence of sodium selenite. For best results, subculture enriched broths within 18 hours to prevent overgrowth or toxicity effects. Always record batch numbers and expiration dates for traceability.
Regulatory and Safety Information
This medium is intended for professional laboratory use only. Sodium selenite is toxic if ingested or inhaled and may cause irritation to the skin or eyes. Handle under Biosafety Level 2 (BSL-2) conditions and wear appropriate personal protective equipment (PPE), including gloves, lab coat, and eye protection. In case of contact with skin or eyes, rinse immediately with water and seek medical advice if irritation persists. Refer to the Safety Data Sheet (SDS) for detailed handling, hazard, and disposal information. BD ensures compliance with global safety and environmental regulations for microbiological reagents.
How to Order
To purchase the BD 221021 BBL Selenite-F Broth 8mL Tubes, add the product to your cart and complete the secure checkout process on the CIA Medical website. For institutional or bulk orders, contact CIA Medical customer service at (312) 275-5850 or submit an inquiry via the online form. CIA Medical provides competitive pricing, fast delivery, and expert support for all BD BBL and Difco microbiological enrichment media products.
