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BD 221832 - Middlebrook Broth with Glycerol Enriched Liquid Medium for Mycobacteria Cultivation
The BD 221832 Middlebrook Broth with Glycerol is a specialized liquid culture medium formulated for the cultivation of Mycobacterium species, including Mycobacterium tuberculosis. This enriched broth provides essential nutrients and growth factors required for the recovery and proliferation of slow-growing mycobacteria.
Incorporating glycerol as a carbon source, this medium supports enhanced growth and metabolic activity. Its liquid format allows for efficient inoculation, uniform distribution of organisms, and easy observation of turbidity, making it ideal for both diagnostic and research applications.
Key Benefits
Enriched formulation supports growth of fastidious mycobacteria.
Glycerol enhances bacterial growth and metabolic activity.
Liquid medium allows uniform culture and easy observation.
Consistent formulation ensures reliable and reproducible results.
Compatibility and Applications
BD Middlebrook Broth with Glycerol is compatible with standard laboratory equipment, including incubation systems and mycobacterial testing workflows.
Common applications include cultivation of Mycobacterium species, tuberculosis diagnostics, antimicrobial susceptibility testing, and microbiological research. It is widely used in hospitals, public health laboratories, and research institutions.
Usage Guidelines
Reconstitute the dehydrated medium with distilled or deionized water according to manufacturer instructions and sterilize as recommended.
Inoculate the broth with the test organism using aseptic technique.
Incubate under appropriate conditions and monitor for turbidity or growth over time.
Certifications and Safety Standards
The BD 221832 Middlebrook Broth with Glycerol is manufactured under ISO-certified quality systems, ensuring consistent performance and reliability.
The product meets established standards for microbiological media, supporting safe and effective use in professional laboratory environments.
Comparison with Competitors
Compared to general-purpose broths, Middlebrook formulations provide superior nutrient support for mycobacteria.
BDs stringent manufacturing processes ensure consistent composition and performance, whereas lower-quality alternatives may not support optimal growth.
The trusted BD brand ensures dependable results, making this medium a preferred choice for mycobacterial culture.
Typical User Profile and Application Scenarios
This product is intended for microbiologists, laboratory technicians, and clinical diagnosticians involved in mycobacterial testing and research.
It is commonly used in hospital laboratories, tuberculosis testing centers, and research facilities where accurate cultivation of Mycobacterium species is required.
Best Practices for Use and Maintenance
Store the dehydrated medium in a cool, dry environment to maintain stability.
Prepare only the required quantity to ensure freshness and reduce waste.
Use aseptic techniques during preparation and inoculation to prevent contamination.
Regulatory and Safety Information
The BD Middlebrook Broth with Glycerol is intended for laboratory use and complies with applicable standards for microbiological media.
Users should follow all relevant biosafety procedures when handling mycobacterial cultures and dispose of materials in accordance with local regulations.
How to Order
To order the BD 221832 Middlebrook Broth with Glycerol, add the product to your cart and proceed through CIA Medicals secure checkout process.
For bulk orders, institutional pricing, or additional assistance, contact CIA Medical customer service at (312) 275-5850. Their team is available to support your procurement needs.
BD #220908, Lowenstein-Jensen Medium (SLA) Enriched Solid Medium for Mycobacterium Isolation and Culture
$50.37 per BOX
BD #212240, Middlebrook OADC Enrichment, 100 mL Supplement for Mycobacterial Culture Media
$1,094.68 per BOX
BD #220908, BBL Tube, Size A, Loewenstein-Jensen Medium, 10 slants/pk (Temp Sensitive; Non-Returnable) (Continental US Only)
$54.44 per PACK