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BD 221263 - Columbia Agar with 5% Sheep Blood Enriched Medium for Fastidious Organism Isolation
The BD 221263 Columbia Agar with 5% Sheep Blood is a high-performance enriched microbiological medium designed for the isolation and cultivation of fastidious microorganisms. This medium combines the nutrient-rich Columbia agar base with 5% defibrinated sheep blood, providing essential growth factors and enabling the observation of hemolytic reactions.
Widely used in clinical microbiology, this medium supports the growth of a broad range of organisms, including Streptococcus, Staphylococcus, and other clinically significant bacteria. The inclusion of sheep blood allows for clear differentiation of hemolysis patterns, making it an essential tool for bacterial identification and diagnostic testing.
Key Benefits
Enriched formulation supports growth of fastidious and clinically important microorganisms.
5% sheep blood enables clear visualization of hemolytic reactions for accurate identification.
Provides consistent and reliable performance across routine and specialized laboratory applications.
Ready-to-use or easily prepared medium supports efficient laboratory workflows.
Compatibility and Applications
BD Columbia Agar with 5% Sheep Blood is compatible with standard laboratory equipment, including Petri dishes, incubators, and automated microbiology systems. It integrates seamlessly into routine culture workflows.
Common applications include isolation of pathogenic bacteria, hemolysis observation, clinical diagnostics, and research studies. It is widely used in hospitals, diagnostic laboratories, public health institutions, and research facilities.
Usage Guidelines
Prepare or use the medium according to manufacturer instructions. If using dehydrated base, reconstitute and sterilize before adding sterile sheep blood under controlled conditions.
Pour into sterile Petri dishes and allow to solidify. Inoculate samples using aseptic techniques and incubate under appropriate conditions.
Observe colony morphology and hemolysis patterns to aid in identification. Follow laboratory protocols for interpretation.
Certifications and Safety Standards
The BD 221263 Columbia Agar with 5% Sheep Blood is manufactured under ISO-certified quality systems, ensuring consistent performance, reliability, and high quality.
Each batch undergoes rigorous quality control testing to meet microbiological standards, supporting its use in professional laboratory environments.
Comparison with Competitors
Compared to standard nutrient agar, Columbia Agar with sheep blood provides enhanced growth support for fastidious organisms and allows hemolysis differentiation.
BDs stringent manufacturing processes ensure consistent quality and performance, whereas lower-quality alternatives may compromise growth or hemolysis clarity.
The trusted BD brand ensures dependable results, making this medium a preferred choice for clinical and diagnostic laboratories.
Typical User Profile and Application Scenarios
This product is intended for microbiologists, laboratory technicians, and clinical diagnosticians involved in bacterial culture and identification.
It is commonly used in hospital laboratories, diagnostic centers, and research facilities where accurate detection and differentiation of bacterial species are essential.
Best Practices for Use and Maintenance
Store the medium under recommended conditions to maintain stability and performance. If dehydrated, keep in a cool, dry environment.
Use aseptic techniques during preparation and inoculation to prevent contamination.
Monitor incubation conditions carefully to ensure optimal growth and accurate interpretation of hemolysis patterns.
Regulatory and Safety Information
The BD Columbia Agar with 5% Sheep Blood is intended for laboratory use and complies with applicable standards for microbiological media.
Users should follow all relevant biosafety procedures when handling microbial cultures and dispose of used media in accordance with local regulations.
How to Order
To order the BD 221263 Columbia Agar with 5% Sheep Blood, add the product to your cart and proceed through CIA Medicals secure checkout process.
For bulk orders, institutional pricing, or additional assistance, contact CIA Medical customer service at (312) 275-5850. Their team is available to support your procurement needs.
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